Table II. Average oxidation indexes (ftsh4/wild type) for selected mitochondrial proteins in all tested experimental setups (LD at 30°C for ftsh4-1 and ftsh4-2 and SD at 22°C for ftsh4-1)

Spot quantification (percentage volume of each spot) was performed on two-dimensional OxyBlots from wild-type and ftsh4 mitochondria using ImageJ Fiji software and on 2D-DIGE gels using DeCyder 2D 6.5 software. The oxidation fold was normalized by the corresponding protein fold to assess the oxidation index.

Protein Spot NumberAccession Number (TAIR)Name of the ProteinOxidation Index
LD at 30°C, ftsh4-1/wild type
 29AT3G10920.2Manganese superoxide dismutase1 (MSD1)17.69
 24AT1G48030.1Mitochondrial lipoamide dehydrogenase1 (MTLPD1)8.29
 5AT3G02090.1Mitochondrial processing peptidase subunit β (MPPBETA)7.29
 20AT1G11860.1Aminomethyltransferase (GDC-T)6.33
 17AT2G47510.1Fumarase (FUM1)5.68
 19AT1G11860.1Aminomethyltransferase (GDC-T)5.11
 18AT3G15020.2Malate dehydrogenase (MDH2)4.95
 16AT2G20420.1Succinyl-CoA ligase subunit β4.32
 21AT4G37930.1Ser hydroxymethyltransferase1 (SHM1)4.02
 6ATMG01190.1ATP synthase subunit α (ATP1)3.47
 10AT2G21870.1ATP synthase subunit Fad (MGP1)2.72
 9AT5G08670.1, AT5G08690.1, AT5G08680.1ATP synthase subunit β (ATP2)2.13
 26AT5G18170.1Glu dehydrogenase1 (GDH1)1.76
 28AT3G61440.1Cys synthase (ATCYSC1)1.72
 13AT2G44350.1Citrate synthase (ATCS)1.58
 4AT5G66760.1Succinate dehydrogenase flavoprotein subunit (SDH1-1)1.09
 2AT1G47260.1γ-Carbonic anhydrase2 (CA2)1.00
LD at 30°C, ftsh4-2/wild type
 14AT4G26970.1Aconitate hydratase2 (ACO2)14.40
 8AT5G08670.1ATP synthase subunit β (ATP2)8.35
 15AT4G26970.1Aconitate hydratase2 (ACO2)6.67
 9AT5G08670.1, AT5G08690.1, AT5G08680.1ATP synthase subunit β (ATP2)4.41
 5AT3G02090.1Mitochondrial processing peptidase subunit β (MPPBETA)4.02
 1AT5G37510.2NADH dehydrogenase (ubiquinone), 75-kD subunit3.83
 21AT4G37930.1Ser hydroxymethyltransferase1 (SHM1)3.29
 6ATMG01190.1ATP synthase subunit α (ATP1)3.21
 22AT4G37930.1Ser hydroxymethyltransferase1 (SHM1)2.96
 29AT3G10920.2Manganese superoxide dismutase1 (MSD1)2.73
 17AT2G47510.1Fumarase (FUM1)2.61
 7ATMG01190.1ATP synthase subunit α (ATP1)2.53
 16AT2G20420.1Succinyl-CoA ligase subunit β2.32
 24AT1G48030.1Mitochondrial lipoamide dehydrogenase1 (MTLPD1)1.76
 19AT1G11860.1Aminomethyltransferase (GDC-T)1.67
 30AT5G15090.1Voltage-dependent anion-selective channel3 (VDAC3)1.63
SD at 22°C, ftsh4-1/wild type
 14AT4G26970.1Aconitate hydratase2 (ACO2)14.62
 15AT4G26970.1Aconitate hydratase2 (ACO2)11.99
 28AT3G61440.1Cys synthase (ATCYSC1)9.40
 1AT5G37510.2NADH dehydrogenase (ubiquinone), 75-kD subunit7.21
 22AT4G37930.1Ser hydroxymethyltransferase1 (SHM1)6.08
 26AT5G18170.1Glu dehydrogenase1 (GDH1)5.50
 24AT1G48030.1Mitochondrial lipoamide dehydrogenase1 (MTLPD1)4.28
 17AT2G47510.1Fumarase (FUM1)4.15
 7ATMG01190.1ATP synthase subunit α (ATP1)3.49
 19AT1G11860.1Aminomethyltransferase (GDC-T)3.41
 16AT2G20420.1Succinyl-CoA ligase subunit β2.95
 21AT4G37930.1Ser hydroxymethyltransferase1 (SHM1)2.71
 8AT5G08670.1ATP synthase subunit β (ATP2)2.41
 25AT5G14780.1Formate dehydrogenase (FDH)1.82
 5AT3G02090.1Mitochondrial processing peptidase subunit β (MPPBETA)1.66
 9AT5G08670.1, AT5G08690.1, AT5G08680.1ATP synthase subunit β (ATP2)1.63
 29AT3G10920.2Manganese superoxide dismutase1 (MSD1)1.23