Table II.

Characterization of TPIs from the wild-type genotype

The assignment of the amino acid sequence of TPI peptides was based on N-terminal sequencing. The molecular mass was determined by MALDI-TOF mass spectrometry, and the predicted mass was calculated from cDNA sequence. The core sequence corresponds to [RIC…CPR] residues of the individual TPI domains (Fig. 3). The analyzed TPI fractions (see Fig. 2 for numbering) were isolated from unelicited plants (control) or plants elicited with MeJA.

FractionMass ObservedMass CalculatedN-Terminal SequenceTPI DomainAssigned Structure
DD
Control 157585758DRIXTBD[core]S
5759DRIXTDD[core]
Control 256705671DRIXTBD[core]
57275729DRIXTFD[core]
57485744DRIXTC or ED[core]
Control 358335831DRIXTCD[core]S
5831DRIXTFD[core]T
Control 457435743DRIXTC or ED[core]
58455845DRIXTED[core]T
5847DD[core]S
Control 556465644RIXTND[core]
5643RIXTNB[core]S
57605760DRIXTDD[core]
5759DRIXTBD[core]S
Control 66142AXPRNAn.a.a
6265KAXPRAn.a.
Control 75130–6265AXPRN/KAXPRAn.a.
MeJA 162316226EKKNDFEEKKND[core]
63326330EKKNDCEKKND[core]S
6330FEKKND[core]T
63916388EEKKNDEEKKND[core]
6387BEEKKND[core]S
64606459EEKKNCEEKKND[core]S
6459FEEKKND[core]T
MeJA 255575556RIXTNB[core]
56175614RIXTNF[core]
56725671DRIXTBD[core]
57305729RIXTNE[core]T
5731RIXTND[core]S
5729DRIXTFD[core]
57615760DRIXTDD[core]
5759DRIXTBD[core]S
58315830DRIXTCD[core]S
5830FD[core]T
63726372EEKKNC or EEEKKND[core]
64756475EEKKNDEEKKND[core]S
6477EEKKNEEEKKND[core]T
MeJA 358345831DRIXTCD[core]S
5831FD[core]T
MeJA 456305628RIXTNC or E[core]
57425743DRIXTC or ED[core]
MeJA 556295628RIXTNC or E[core]
57425743DRIXTC or ED[core]
58465845DRIXTED[core]T
5847DD[core]S
57335729DRIXTFD[core]
5730RIXTNE[core]T
5732D[core]S
MeJA 656465644RIXTND[core]
5643B[core]S
57615760DRIXTDD[core]
5759BD[core]S
MeJA 76137AXPRNAn.a.
6265KXPRAn.a.
MeJA 85130–6265KAXPR/AXPRNAn.a.
  • a n.a., Not assigned.