Table IV.

Characterization of TPIs from S++ plants in which the TPI production of the Arizona genotype was restored by transformation

The assignment of the amino acid sequence of the TPI peptides is based on N-terminal sequencing. The molecular mass was determined by MALDI-TOF mass spectrometry, and the predicted mass was calculated from cDNA sequence. The core sequence corresponds to [RIC…CPR] residues of the individual TPI domains (Fig. 3). The analyzed TPI fractions (see Fig. 10 for numbering) were isolated from unelicited plants (control) or plants elicited with MeJA.

FractionMass ObservedMass CalculatedN-Terminal SequenceTPI DomainAssigned Structure
DD
Control 156735671DRIXTBD[core]
57325729DRIXTFD[core]
MeJA 156735671DRIXTBD[core]
57315729DRIXTFD[core]
Control 258325831DRIXTCD[core]S
5831DRIXTFD[core]T
MeJA 258325831DRIXTCD[core]S
5831DRIXTFD[core]T
Control 356335628RIXTC or E[core]
57485743DRIXTC or ED[core]
58415844DRIXTED[core]T
5846DD[core]S
MeJA 356315628RIXTC or E[core]
57465743DRIXTC or ED[core]
58485844DRIXTED[core]T
5846DD[core]S
Control 456445644RIXTND[core]
5643RIXTNB[core]S
57595760DRIXTDD[core]
5759DRIXTBD[core]S
MeJA 456465644RIXTND[core]
5643RIXTNB[core]S
57625760DRIXTDD[core]
5759DRIXTBD[core]S
Control 56140AXPRNAn.a.a
6270KAXPRAn.a.
MeJA 56138AXPRNAn.a.
6269KAXPRAn.a.
  • a n.a., Not assigned.