Table I.

Biological and analytical variation of the tomato volatile metabolites

For the analysis, a mix of tomato samples was made and separate aliquots were measured after 0, 4, 8, and 12 h. Using these four measurements, % sd (presented as the % of total value) was calculated for the sole use of CaCl2 (second column) and for the combination NaOH/EDTA + CaCl2 (third column). For the analysis of biological variation within genotype (fourth column), five individual fruits of the same genotype were profiled for volatiles, and % sd for these five replicates was calculated. Biological variation between genotypes (fifth column) was calculated as % sd of means of all 94 tomato samples when NaOH/EDTA + CaCl2 procedure was used. The maximal relative fruit-to-fruit variation as well as the maximal variation between all 94 genotypes was calculated as the ratio of maximal and minimal relative values of the 15 volatiles across the five fruits and the 94 genotype samples, respectively. It is given in parenthesis as fold difference (fourth and fifth columns).

MetabolitesAnalytical Variation, % sdBiological Variation within Genotype n=5, % sdBiological Variation between Genotypes n=94, % sd
CaCl2NaOH/EDTA + CaCl2
1-Penten-3-one19715 (1.4)45 (6)
2-Isobutylthiazole39413 (1.4)64 (47)
2-Methylbutanal881134 (2.7)60 (16)
2-Methylbutanol38835 (2.5)76 (39)
3-Methylbutanol37728 (2.2)74 (29)
6-Methyl-5-hepten-2-one33417 (1.5)37 (7)
β-Ionone511710 (1.3)62 (14)
E-2-Heptenel3888 (1.2)39 (10)
E-2-Hexenal34525 (1.8)37 (6)
Hexanal17310 (1.3)29 (8)
Methyl salicylate49921 (1.6)173 (656)
Phenylacetaldehyde42623 (1.8)162 (401)
Phenylethanol48419 (1.5)198 (686)
Z-3-Hexenal402323 (1.7)28 (7)
Z-3-Hexenol391317 (1.5)103 (28)
Average4191879