E3-E2 specificity as determined by in vitro ubiquitination assays
Each E3 or GST-RING protein was tested in in vitro ubiquitination assays with a number of Arabidopsis E2s from different subfamilies as described and defined by Stone et al. (2005). Protein ubiquitination was observed (+) for a number of E2-E3 combinations. Two instances occurred where activity was inconsistent (±). For other E2-E3 combinations, protein ubiquitination was not detected (−). E2-E3 combinations that were not determined (nd) are indicated.
| E3 | RING Type | E2 (UBC) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 8 | 10 | 11 | 28 | 29 | 30 | 19 | 34 | 35 | 36 | ||
| At1g02860 | HCa | − | − | − | − | − | − | − | − | − | − |
| At1g12760 | H2 | + | + | + | + | + | − | − | − | − | − |
| At1g14260 | v | + | + | + | + | − | − | − | − | − | − |
| At1g18760 | D | + | + | + | + | − | − | − | − | − | − |
| At1g22500 | H2 | + | + | + | + | + | − | − | − | − | − |
| At1g50440 | v | + | + | + | − | − | ± | − | − | − | − |
| At1g61620 | HCa | − | − | − | − | − | − | − | − | − | − |
| At1g63900 | HCa | + | + | + | + | + | − | − | − | − | − |
| At1g65430 | HCb | + | + | + | − | − | − | − | − | − | − |
| At1g68180 | H2 | + | + | + | − | + | − | − | + | nd | − |
| At1g74410 | H2 | − | − | − | − | − | − | − | − | − | − |
| At2g18670 | H2 | − | − | − | − | − | − | − | − | − | − |
| At2g22690 | S/T | − | − | − | − | − | − | − | − | − | − |
| At2g28530 | C2 | + | − | + | − | − | − | − | − | nd | − |
| At2g28840 | HCa | − | − | − | − | − | − | − | − | − | − |
| At2g42360 | H2 | + | + | + | + | + | + | − | − | + | + |
| At2g44330 | H2 | + | + | + | + | + | + | − | − | − | − |
| At2g47700 | H2 | + | + | + | + | + | − | − | − | + | + |
| At3g05250 | HCa | + | + | + | + | − | − | − | − | − | − |
| At3g05545 | H2 | + | ± | + | − | − | − | − | − | − | − |
| At3g06330 | v | + | + | + | + | + | − | − | − | − | − |
| At3g09760 | v | + | + | + | − | − | − | − | − | nd | − |
| At3g29270 | HCa | + | + | + | + | + | − | − | − | − | − |
| At3g45555 | HCb | − | − | − | − | − | − | − | − | nd | − |
| At3g47160 | HCa | + | + | + | − | − | − | − | − | − | − |
| At3g48070 | C2 | + | + | + | − | − | − | − | − | nd | − |
| At4g10160 | H2 | + | + | + | − | − | − | − | + | nd | − |
| At4g14220 | H2 | + | + | + | + | + | − | − | − | + | + |
| At4g27470 | HCa | + | + | + | + | + | − | − | − | − | − |
| At5g20910 | H2 | + | + | + | + | + | + | − | − | − | − |
| At5g37270 | H2 | + | + | + | + | + | − | − | − | + | + |
| At5g38070 | v | + | + | + | − | − | − | − | − | − | − |
| At5g42200 | H2 | + | + | + | + | + | − | − | − | − | − |
| At5g53910 | D | + | + | + | − | − | − | − | − | nd | − |