Table II.

Identification of embryo sac genes

Genes were selected on the basis of the P value of <0.0050, fold change of >2.0, and signal intensity of >60.0 at wild-type siblings. WT1–3, Mean of signal intensities for arrays 1 to 3 of wild-type siblings. spl1–3, Mean of signal intensities for arrays 1 to 3 of spl mutants. WTd1–d3, Mean of signal intensities for arrays 1 to 3 of emasculated wild-type siblings. spl1–3, d1–d2, Mean of signal intensities for arrays 1 to 3 of nonemasculated spl, and arrays 1 and 2 of emasculated spl.

Affymetrix CodeaGene IDbDescriptionEarly Staged OvuleLate Staged OvuleDivisione
WT1–3spl1–3FCcP ValuedWTd1–d3spl1–3, d1–d2FCcP Valued
Unknown protein
    261271_atAt1g26795Self-incompatibility protein related7693025.30.0000837612728.20.000000Both
    265133_s_atAt1g51250Expressed protein151256.10.0014565391928.20.000000Both
    259726_atAt1g60985Expressed protein98165.90.0021403491621.40.000003Both
    265762_atAt2g01240Reticulon family protein (RTNLB15)2271515.10.0004181481410.30.000020Both
    263713_atAt2g20595Expressed protein2781518.90.0035561,0472541.40.000000Both
    267193_atAt2g30900Expressed protein97234.30.000554108205.40.000016Both
    256719_atAt2g34130CACTA-like transposase family (Ptta/En/Spm)1171110.30.0001811141011.80.000000Both
    256600_atAt3g14850Expressed protein261347.60.000088294358.50.000000Both
    257889_atAt3g17080Self-incompatibility protein related187306.20.0029313421917.80.000000Both
    251698_atAt3g56610Expressed protein5181632.00.0046821,4443146.80.000000Both
    254257_s_atAt4g23350Expressed protein104303.50.0038793352215.30.000000Both
    253164_atAt4g35725Expressed protein1581510.40.0030442981421.20.000024Both
    250871_atAt5g03930Expressed protein171247.00.001971106147.50.000002Both
    250325_s_atAt5g12060Self-incompatibility protein related138413.40.001255206306.80.000001Both
    249855_atAt5g22970Expressed protein2612510.60.0035171,0283231.70.000001Both
    249757_atAt5g24316Pro-rich family protein4711727.70.0017661,3282945.60.000000Both
    249401_atAt5g40260Nodulin MtN3 family protein5663715.20.0006034273014.30.000004Both
    248284_atAt5g52975Expressed protein755819.30.000353403498.20.000001Both
    262503_atAt1g21670Expressed protein161414.00.00000964451.40.031616Early
    261731_s_atAt1g47780Acyl-protein thioesterase related84155.60.00414060134.70.000003Early
    249375_atAt5g40730Arabinogalactan protein (AGP24)8061296.20.0003426615751.10.245297Early
    264610_atAt1g04645Self-incompatibility protein related106185.70.1579507932138.10.000557Late
    261846_atAt1g11540Expressed protein1451071.40.117890170852.00.000012Late
    255908_s_atAt1g18010Expressed protein128642.00.01805893452.10.000005Late
    256079_atAt1g20680Expressed protein56143.90.01179867125.70.000019Late
    263027_atAt1g24010Expressed protein23121.90.25658096224.30.000001Late
    260942_s_atAt1g45190Expressed protein318649.30.0074971,4043047.10.000000Late
    265138_atAt1g51300Acyl-protein thioesterase related192434.40.015275450499.10.000000Late
    262314_atAt1g70810C2 domain-containing protein23171.40.18277565173.80.000004Late
    262972_atAt1g75620Glyoxal oxidase related67491.40.205253133383.50.000079Late
    264297_atAt1g78710Expressed protein120621.90.005001144572.50.000001Late
    267241_atAt2g02490Hydroxyproline-rich glycoprotein family protein45410.60.0524663951137.00.000186Late
    267218_atAt2g02515Expressed protein163394.20.0072068383424.80.000001Late
    265517_atAt2g06090Self-incompatibility protein related2541417.80.0139731,1092347.40.000000Late
    264590_atAt2g17710Expressed protein1441940.70.3613478413942.10.000010Late
    263518_atAt2g21655Expressed protein2711715.80.0154129462537.60.000001Late
    265674_atAt2g32190Expressed protein100611.60.072973198702.80.000267Late
    265670_s_atAt2g32210Expressed protein1551581.00.888298213762.80.000186Late
    265245_atAt2g43060Expressed protein39450.90.564913131622.10.000340Late
    259107_atAt3g05460Sporozoite surface protein related403439.40.0083481,4154631.10.000069Late
    258130_atAt3g24510Expressed protein4861048.60.0129872,0333853.80.000000Late
    252253_atAt3g49300Pro-rich family protein251426.00.0077546263219.30.000044Late
    251606_atAt3g57840Self-incompatibility protein related109392.80.0583914683812.40.000005Late
    255207_atAt4g07515Expressed protein4532319.80.0082758593227.00.000000Late
    245424_atAt4g17505Expressed protein157552.80.005099312339.40.000003Late
    254494_atAt4g20050Expressed protein125851.50.069310230593.90.000001Late
    254001_atAt4g26260Expressed protein174622.80.067591149255.90.000073Late
    253724_atAt4g29285Expressed protein91127.30.0290366471639.50.000000Late
    253656_atAt4g30090Expressed protein101851.20.27635876372.10.000137Late
    253401_atAt4g32870Expressed protein88412.10.040870165394.30.000012Late
    246641_s_atAt5g34885Expressed protein2162110.30.0252296312723.50.000000Late
    249179_atAt5g42955Expressed protein2661419.30.0148618822338.00.000000Late
    248892_atAt5g46300Expressed protein62511.80.014887124523.10.000001Late
Central intermediary metabolism
    259786_atAt1g29660GDSL-motif lipase/hydrolase family protein289843.40.0031668794252.10.000131Both
    260124_atAt1g36340Ubiquitin-conjugating enzyme, E25843815.30.0016614673413.80.000002Both
    245672_atAt1g56710Glycoside hydrolase family 28 protein218852.60.004220235942.50.000298Both
    257442_atAt2g28680Cupin family protein316476.80.001424303339.20.000008Both
    267408_atAt2g34890CTP synthase, putative152256.10.00065167164.20.000011Both
    257243_atAt3g24230Pectate lyase family protein332595.70.003792198633.20.000001Both
    258763_s_atAt3g30540(1-4)-β-Mannan endohydrolase family125815.90.002254148917.40.000000Both
    252342_atAt3g48950Glycoside hydrolase family 28 protein3483011.60.0015766032623.30.000000Both
    248925_atAt5g45910GDSL-motif lipase/hydrolase-like protein2791420.00.0016131,3672653.50.000000Both
    247228_atAt5g65140Trehalose-6-phosphate phosphatase305388.00.000647246922.70.000044Both
    264147_atAt1g02200CER1 protein206852.40.00020540391.00.799875Early
    264146_atAt1g02205CER1 protein, At1g022006502792.30.0000212192251.00.820363Early
    259703_atAt1g77790Glycosyl hydrolase family 17 protein113167.00.00042854114.80.000000Early
    267202_s_atAt2g31030Oxysterol-binding family protein74107.30.0008642773.80.000005Early
    260611_atAt2g43670Glycosyl hydrolase family 17 protein132532.50.002326129751.70.000212Early
    252320_atAt3g48580Xyloglucan:xyloglucosyl transferase, putative7433152.40.0037951,1767661.50.004917Early
    250082_atAt5g17200Glycoside hydrolase family 28 protein71174.20.00291020111.80.000578Early
    260947_atAt1g06020PfkB-type carbohydrate kinase family protein58401.50.01046375292.60.000134Late
    259391_s_atAt1g06350Fatty acid desaturase family protein163981.70.342723196882.20.000376Late
    255956_atAt1g22015Galactosyltransferase family protein158433.70.007113274348.00.000000Late
    245792_atAt1g32100Pinoresinol-lariciresinol reductase, putative66661.00.993453184682.70.000127Late
    245794_atAt1g32170Xyloglucan:xyloglucosyl transferase, putative59491.20.363717101472.10.000150Late
    256211_atAt1g50960Gibberellin 20-oxidase related42192.20.01534075145.40.000000Late
    260333_atAt1g70500Polygalacturonase, putative/pectinase, putative5812721.70.0079596744216.00.000001Late
    260066_atAt1g73610GDSL-motif lipase/hydrolase family protein69242.90.035163163208.00.000056Late
    260259_atAt1g74300Esterase/lipase/thioesterase family protein60571.10.690243105472.20.000095Late
    265331_atAt2g18420Gibberellin-responsive protein, putative27251.10.77795373184.10.000139Late
    267607_s_atAt2g26740Epoxide hydrolase, soluble (sEH)1381371.00.977676205872.40.000242Late
    267337_atAt2g39980Transferase family protein93462.00.01525169302.30.000006Late
    260559_atAt2g43860Polygalacturonase, putative/pectinase, putative113383.00.00592069302.30.000032Late
    258767_atAt3g10890(1-4)-β-Mannan endohydrolase, putative100166.30.0137536982035.10.000002Late
    258151_atAt3g18080Glycosyl hydrolase family 1 protein1991941.00.8999353841243.10.000003Late
    257065_atAt3g18220Phosphatidic acid phosphatase family protein45291.50.03402063222.80.000003Late
    251491_atAt3g59480PfkB-type carbohydrate kinase family protein549569.90.0327825982102.80.001882Late
    255550_atAt4g01970Galactinol-raffinose galactosyltransferase, putative127413.10.006794226832.70.000170Late
    245349_atAt4g16690Esterase/lipase/thioesterase family protein75401.90.193063112532.10.000368Late
    254609_atAt4g18970GDSL-motif lipase/hydrolase family protein4615080.90.1899427033092.30.000085Late
    246498_atAt5g16230Acyl-[acyl-carrier-protein] desaturase, putative34261.30.21992984233.60.001229Late
    249983_atAt5g18470Curculin-like (mannose-binding) lectin family protein44351.20.486888106482.20.001507Late
    246774_atAt5g27530Glycoside hydrolase family 28 protein108462.40.012277146314.80.000001Late
    249474_s_atAt5g39190Germin-like protein (GER2)14121.20.50073299137.60.000004Late
    248812_atAt5g47330Palmitoyl protein thioesterase family protein63780.80.143704203434.70.000004Late
    248791_atAt5g47350Palmitoyl protein thioesterase family protein212653.30.0406441,1672914.00.000003Late
Hypothetical protein
    260318_atAt1g63960Hypothetical protein62203.20.00292472203.50.000014Both
    265579_atAt2g20070Hypothetical protein6614813.80.0000414873613.40.000000Both
    257434_atAt2g21740Hypothetical protein128255.00.0012383012015.30.000000Both
    252753_atAt3g43500Hypothetical protein80204.00.002002131206.60.000002Both
    246859_atAt5g25950Hypothetical protein70135.40.0000451311210.90.000000Both
    248225_atAt5g53740Hypothetical protein168722.30.000851111522.10.000065Both
    266706_atAt2g03320Hypothetical protein97283.50.00111647172.80.000175Early
    246866_atAt5g25960Hypothetical protein1311111.70.0000004194.80.000020Early
    248396_atAt5g52130Hypothetical protein111215.40.00060947153.20.000216Early
    257468_atAt1g47470Hypothetical protein1471311.20.0292731,0102540.10.000000Late
    261313_atAt1g52970Hypothetical protein484499.90.0079051,8815932.10.000000Late
    259944_atAt1g71470Hypothetical protein72601.20.21312171332.10.000104Late
    263895_atAt2g21920Hypothetical protein107264.20.00754273174.20.000906Late
    258798_atAt3g04540Hypothetical protein202553.70.0870471,5425030.90.000000Late
    256773_atAt3g13630Hypothetical protein31281.10.71567760272.20.000839Late
    251607_atAt3g57850Hypothetical protein73116.60.0360563701329.40.000050Late
    255029_x_atAt4g09470Hypothetical protein139817.20.0127146871643.50.000003Late
    255804_atAt4g10220Hypothetical protein120313.90.0096524103412.00.000003Late
    254692_atAt4g17860Hypothetical protein60351.70.028983107283.90.000008Late
    246472_atAt5g17130Hypothetical protein80831.00.85323375342.20.000487Late
    249157_atAt5g43510Hypothetical protein3561327.30.0121785882920.10.000001Late
    247245_atAt5g64720Hypothetical protein105156.90.0103923221324.50.000000Late
Detoxification/stress response
    264001_atAt2g22420Peroxidase 17 (PER17)7911216.50.0037187251206.10.000007Both
    265264_atAt2g42930Glycosyl hydrolase family protein 17346556.30.003492285348.50.000007Both
    254000_atAt4g26250Galactinol synthase induced by water stress89184.80.00389265154.20.000004Both
    252951_atAt4g38700Disease resistance-responsive family protein2481092.30.0042287061156.20.000000Both
    247857_atAt5g58400Peroxidase, putative1,0532404.40.0000044731612.90.000023Both
    261410_atAt1g07610Metallothionein-like protein 1C (MT-1C)3331592.10.0008322381701.40.168590Early
    266743_atAt2g02990Ribonuclease 1 (RNS1)67232.90.0013541401001.40.201557Early
    263026_atAt1g24000Bet v I allergen family protein43152.90.2662782501912.90.000001Late
    265920_s_atAt2g15120Pseudogene, disease-resistance family protein38331.10.299880103234.60.000933Late
    266562_atAt2g23970Defense-related protein, putative68601.10.479766194385.10.000000Late
    267138_s_atAt2g38210Ethylene-responsive protein, putative181862.10.091609246763.20.001233Late
    266169_atAt2g38900Ser protease inhibitor40371.10.8673133431053.30.000002Late
    260557_atAt2g43610Glycoside hydrolase family 19 protein94491.90.00326176332.30.000002Late
    258791_atAt3g04720Hevein-like protein (HEL)35351.00.93736991342.60.003414Late
    258172_atAt3g21620Early responsive to dehydration protein related60242.50.00059768213.20.000002Late
    254098_atAt4g25100Superoxide dismutase (Fe), chloroplast (SODB)6883132.20.06051876145.60.000220Late
    253655_atAt4g30070Plant defensin-fusion protein, putative99392.50.007716167237.40.000001Late
    250200_atAt5g14130Peroxidase, putative94671.40.229669106412.60.000012Late
    250083_atAt5g17220Glutathione S-transferase-like protein99931.10.8709005372352.30.000020Late
    249560_atAt5g38330Plant defensin-fusion protein, putative2802611.00.0084351,1402939.50.000000Late
    249527_atAt5g38710Pro oxidase, putative70183.90.00617674154.80.000019Late
Cell structure organization
    260573_atAt2g47280Pectinesterase family protein227366.40.000454139255.50.000000Both
    257878_atAt3g17150Invertase/pectin methylesterase inhibitor family3992615.30.0043751,1213829.20.000000Both
    258438_atAt3g17230Invertase/pectin methylesterase inhibitor family4343014.40.0009247583024.80.000000Both
    251748_atAt3g55680Invertase/pectin methylesterase inhibitor family162513.20.000901112392.80.000002Both
    255699_atAt4g00190Putative pectinesterase86312.80.00153790243.70.000001Both
    248823_s_atAt5g46960Invertase/pectin methylesterase inhibitor family270835.10.0028905191339.40.000003Both
    247246_atAt5g64620Invertase/pectin methylesterase inhibitor family7473382.20.0002988704252.00.000000Both
    260802_atAt1g78400Glycoside hydrolase family 28 protein201238.80.00000130181.70.001391Early
    258764_atAt3g10720Pectinesterase, putative4152052.00.001974112771.50.008263Early
    253725_atAt4g29340Profilin 3 (PRO3) (PFN3)63272.30.00044626231.10.244366Early
    264500_atAt1g09370Pectinesterase inhibitor domain-containing protein61106.00.1239495941443.80.000012Late
    259613_atAt1g48010Invertase/pectin methylesterase inhibitor family38361.10.597625105293.60.000000Late
    262083_atAt1g56100Pectinesterase inhibitor domain-containing protein128532.40.4446292,6227463.50.000012Late
    245656_atAt1g56620Pectinesterase inhibitor domain-containing protein50261.90.0442752521913.30.000000Late
    257679_atAt3g20470Pseudogene, Gly-rich protein117991.20.7003085782672.20.001050Late
    245371_atAt4g15750Invertase/pectin methylesterase inhibitor family200633.20.0674541,3784403.10.000004Late
    249962_atAt5g18990Pectinesterase family protein39192.00.02824582155.50.000041Late
    247377_atAt5g63180Pectate lyase family protein1751251.40.1472624101922.10.000518Late
Transport
    260319_atAt1g63950Heavy-metal-associated domain-containing protein3102810.90.002905148197.90.000001Both
    259757_atAt1g77510Protein disulfide isomerase, putative1,1634472.60.0003449433672.60.000170Both
    258760_atAt3g10780Emp24/gp25L/p24 family protein435944.60.001770400755.40.000000Both
    245892_atAt5g09370Protease inhibitor/seed storage/lipid transfer protein384576.80.0046301,32610812.30.000002Both
    263765_atAt2g21540SEC14 cytosolic factor, putative135642.10.00098564421.50.002042Early
    249346_atAt5g40780Lys- and His-specific transporter, putative7831704.60.0025998776241.40.054790Early
    264520_atAt1g10010Amino acid permease, putative91352.60.005939167344.80.000000Late
    265002_atAt1g24400Lys- and His-specific transporter156413.80.0249333111422.20.000005Late
    259580_atAt1g28030Oxidoreductase, 2OG-Fe(II) oxygenase family protein52441.20.27655795323.00.000024Late
    265064_atAt1g61630Equilibrative nucleoside transporter, putative (ENT7)70401.70.01322067292.30.000179Late
    259844_atAt1g73560Protease inhibitor/seed storage/lipid transfer protein49222.20.00523491194.90.000122Late
    264482_atAt1g77210Sugar transporter, putative73651.10.601658118552.10.000012Late
    257366_s_atAt2g03040Transmembrane protein related159394.10.005632184228.30.000008Late
    266276_atAt2g29330Tropinone reductase, putative68302.30.00741068282.40.000243Late
    254453_atAt4g21120Amino acid permease family protein211643.30.007991259882.90.000000Late
    246887_atAt5g26250Sugar transporter, putative159612.60.015475146374.00.000013Late
    248275_atAt5g53520Oligopeptide transporter OPT family protein43321.30.20472280203.90.000002Late
    248019_atAt5g56480Protease inhibitor/seed storage/lipid transfer protein61501.20.206709111382.90.000015Late
Protein degradation
    256486_atAt1g31450Aspartyl protease family protein2631814.80.0013116071735.10.000000Both
    245738_atAt1g44130Nucellin protein, putative1171012.30.00298894811.20.000067Both
    259368_atAt1g69100Aspartyl protease family protein5461342.60.0037569523329.10.000000Both
    252499_s_atAt3g46840Subtilase family protein3893411.60.0003984432617.20.000001Both
    245589_atAt4g15040Subtilase family protein171237.30.00015466174.00.000055Both
    254336_atAt4g22050Aspartyl protease family protein6813718.30.0000967146710.60.000000Both
    246684_atAt5g33340Aspartyl protease family protein98254.00.000228189219.10.000001Both
    247798_atAt5g58830Subtilase family protein73155.00.0026732351317.70.000000Both
    254237_atAt4g23520Cys proteinase, putative67282.40.00024341221.90.000254Early
    247697_atAt5g59810Subtilase family protein92432.10.00228856441.30.003872Early
    264067_x_atAt2g28010Aspartyl protease family protein33191.70.03984478135.90.000004Late
    250345_atAt5g11940Subtilase family protein58153.90.0055911541411.40.000000Late
Signal transduction
    263740_atAt2g20660Rapid alkalinization factor (RALF) family protein82332.40.00075890243.80.000000Both
    245158_atAt2g33130RALF family protein4353412.80.0009894373811.40.000000Both
    266418_atAt2g38750Annexin 4 (ANN4)274624.40.003825220673.30.000001Both
    251514_atAt3g59260Pirin, putative202336.10.001924171305.70.000002Both
    255489_atAt4g02650Epsin N-terminal homology domain-containing protein4482220.70.0043571,2863239.80.000000Both
    245177_atAt5g12380Annexin, putative1901711.20.0003932151712.80.000000Both
    249013_atAt5g44700Leu-rich repeat transmembrane protein kinase81312.60.00130375302.50.000305Both
    261285_atAt1g35720Annexin 1 (ANN1)1681221.40.2736973931083.60.000012Late
    257869_atAt3g25160ER lumen protein retaining receptor family protein86511.70.030431147522.80.000020Late
    250090_atAt5g17330Glutamate decarboxylase 1 (GAD 1)195219.20.005864222259.00.000000Late
Energy metabolism
    259268_atAt3g01070Plastocyanin-like domain-containing protein79282.80.002166126264.80.000001Both
    253634_atAt4g30590Plastocyanin-like domain-containing protein7055612.60.0000575004112.30.000005Both
    252897_atAt4g39490Cytochrome P450 family protein, At4g3848062193.40.004614134187.50.000000Both
    248236_atAt5g53870Plastocyanin-like domain-containing protein1,2366020.70.0003946531654.00.000009Both
    262528_atAt1g17260ATPase 10, plasma membrane type, putative45261.70.3857223441023.40.000002Late
    261396_atAt1g79800Plastocyanin-like domain-containing protein49401.20.23612469312.20.000064Late
    266563_atAt2g23990Plastocyanin-like domain-containing protein195474.20.0110179534123.30.000015Late
    255690_atAt4g00360Cytochrome P450, putative49232.10.01830979292.70.000026Late
    254489_atAt4g20800FAD-binding domain-containing protein47114.40.0278031211111.30.000000Late
Transcriptional regulation
    267528_atAt2g45650MADS-box protein (AGL6)245594.20.0029043591702.10.000048Both
    249338_atAt5g41090No apical meristem (NAM) family protein166189.10.00076477135.80.000000Both
    260212_atAt1g74480RWP-RK domain-containing protein55361.50.07115165292.20.001510Late
    266969_atAt2g39540Gibberellin-regulated family protein41381.10.593532179325.60.000003Late
    254619_atAt4g18770Myb family transcription factor (MYB98)109591.80.063530110412.70.000002Late
    251114_atAt5g01380Expressed protein25261.00.85550562232.70.000211Late
    248240_atAt5g53950No apical meristem (NAM) family protein2242011.10.3995442691102.40.000013Late
Secondary metabolism
    260386_atAt1g74010Strictosidine synthase family protein110215.10.0013133682216.40.000010Both
    264401_atAt1g61720Dihydroflavonol 4-reductase family (BAN)54451.20.6935825351763.00.000017Late
    260335_atAt1g74000Strictosidine synthase family protein144722.00.005947104392.70.000038Late
    254283_s_atAt4g22870Leucoanthocyanidin dioxygenase, putative81851.00.8809184161832.30.000118Late
    249215_atAt5g42800Dihydroflavonol 4-reductase31261.20.546725301843.60.000030Late
Plant development/organogenesis
    262113_atAt1g02820Late embryogenesis abundant 3 family protein82332.50.00005776272.80.000001Both
    262659_atAt1g14240Nucleoside phosphatase family protein80253.30.00091192165.80.000000Both
    262549_atAt1g31290PAZ domain-containing protein140991.40.096043169632.70.000011Late
    252234_atAt3g49780Phytosulfokines 3 (PSK3)1792.00.386279163433.80.000014Late
    251301_atAt3g61880Cytochrome P450, putative94511.90.00380991412.20.000032Late
Protein biosynthesis/DNA catabolism
    245883_atAt5g0950040S ribosomal protein S15 (RPS15C)85184.70.0020805495.90.000028Early
    260438_atAt1g68290Bifunctional nuclease, putative44281.60.093917149255.90.000104Late
  • a Affymetrix probe set number.

  • b Arabidopsis Genome Initiative number.

  • c Fold change for signal intensity of wild type/signal intensity of spl.

  • d P values calculated by Student's t test for two-sample equal variance.

  • e Expressed ovule stage.