Table I.

Summary of expression patterns of PRF constructs in reproductive organs

GUS staining intensity in various tissues was indicated by a minus (−), negative or weakly detectable; plus (+), light blue; ++, blue; +++, dark blue. The flower stalks were cut and incubated in GUS staining solution for 12 h. The ratio of the number of transgenic plants that show GUS staining to the total number of independent transgenic plants is indicated below the staining intensity.

ConstructStigmaAntherFilamentPetalSepalCut Edge
pPRF2e+++++
(0/23)(0/23)(21/23)(0/23)(16/23)(16/23)
pPRF2e-p5i+++
(0/23)(0/23)(14/23)(0/23)(7/23)(2/23)
pPRF5e+++++
(12/12)(12/12)(2/12)(1/12)(0/12)(0/12)
pPRF5e-p5i+++++
(10/13)(13/13)(1/13)(1/13)(0/13)(0/13)
pPRF5e-p2i+++++++++++++
(15/24)(21/24)(19/24)(8/24)(15/24)(23/24)