SHMT activity in Arabidopsis leaf extracts
Total SHMT activity was assayed in leaf extracts from the wild type (Col-0), the shm1-1 mutant, two independent primary shm1-1-35S:SHM1 transformants (2T1 and 3T1), homozygous shm1-2, and F1 progeny from a cross between shm1-1 and shm1-2. Results represent the means of 12 replicates ±se that were obtained in four independent experiments.
| Genetic Background | SHMT Activity |
|---|---|
| nmol mg−1 min−1 | |
| Col-0 | 1.64 ± 0.07 |
| shm1-1 | 0.18 ± 0.01 |
| 2T1 | 1.60 ± 0.06 |
| 3T1 | 1.49 ± 0.08 |
| shm1-2 | 0.20 ± 0.02 |
| shm1-2 × shm1-1 | 0.22 ± 0.02 |