Table III.

Effects of leaf aging on the activity of the Fd-Glu synthase promoter in transgenic tobacco plants, which harbor the GLU1 promoter of the Arabidopsis Fd-GOGAT fused to a GUS reporter gene (GLU1::GUS)

In vitro GUS activity was determined in two transgenic lines: GLU1GUS*1 and GLU1GUS*2.

TissuesYoung Leaves and RootsOld Leaves and Roots
GLU1GUS*1GLU1GUS*2GLU1GUS*1GLU1GUS*2
GUS activity: nmol 4-MU mg−1 protein min−1
Leaves
    Mesophyll58.41 ± 4.9014.10 ± 1.2734.05 ± 3.27.86 ± 1.08
    Vein5.02 ± 0.424.55 ± 0.414.67 ± 0.424.01 ± 0.36
    Petiole2.93 ± 0.252.17 ± 0.202.71 ± 0.231.95 ± 0.18
Roots1.22 ± 0.090.45 ± 0.051.16 ± 0.110.41 ± 0.04