Table I.

Overview of the nine ROS-generating systems included in the comprehensive analysis

For each experimental system, the identities of the major generated ROS and the intracellular sites of production are indicated. Sample and treatment descriptions together with other relevant experimental parameters are listed. Expression analysis of all experiments was performed using the Affymetrix ATH1 (ATH1) or Agilent Arabidopsis2 (Arab2) arrays. CAT2HP1, CAT-deficient plants; WT, wild type; KD, knockdown; AS, antisense; Chl, chloroplasts; Mit, mitochondria; Pl, plastids; Cyt, cytosol; Per, peroxisomes; Apo, apoplast. 1, op den Camp et al. (2003); 2, Rizhsky et al. (2003); 3, Umbach et al. (2005); 4, Davletova et al. (2003); 5, Vanderauwera et al. (2005); 6, Gechev et al. (2004); 7, Gechev et al. (2005); 8, D. Bartels (Bonn), AtGenExpress repository; 9, A. Shirras (Lancaster, UK), NASCArray repository.

ExperimentMajor ROSLocalizationPlant AgeSample DescriptionTreatment/Growth ConditionsTime PointsReplicatesMicroarray PlatformSource
weeksh after Treatment
flu mutant1O2Pl3flu mutantDark-to-light shift (80–100 μmol m−2 s−1)0.5, 1, 21ATH11
KD-SODO2·−Chl3Cu/Zn-SOD-KD plantsStandard3ATH12
AOX1a-ASO2·−, H2O2Mit3AOX1a-AS plantsStandard2ATH13
KO-Apx1 + HLH2O2Cyt3KO-Apx1 plantsHL shift: 25 to 250 μmol m−2 s−10, 0.25, 0.5, 1.5, 3, 6, 242ATH14
CAT2HP1 + HLH2O2Per6CAT2HP1 plantsHL shift: 140 to 1,800 μmol m−2 s−10, 3, 82ATH15
LOH2 + AAL toxinH2O24LOH2 plantsWater/AAL toxin injection, 200 nm7, 24, 48, 721Arab26
AT sprayH2O2Per4WT plantsAT spray, 20 mm71Arab27
MVO2·−Mit/Chl2.5WT plantsMV, 10 μmol0.5, 1, 3, 6, 12, 242ATH18
O3 fumigationO3, O2·−, H2O2Apo2WT plantsO3, 500 μg L−163ATH19