Table II.

Endosperm lipid accumulation and hypocotyl elongation

Line 101 and line 106 express the WRI1 cDNA under the control of the 35S-CaMV promoter. F1 seed of reciprocal crosses between wild type and the homozygous wri1-1 mutant were analyzed as well. Fatty acid methyl esters (FAME) of total seeds and endosperm were analyzed. Three samples containing 15 seeds or endosperms each were analyzed and the sd of these three samples is shown. Hypocotyl length was measured on 50 mm Suc (+Suc) or in the absence of Suc (−Suc). The sd of the mean from three replicates is given. Hypocotyl measurements were averaged for 30 to 40 plants per treatment. n.d., Not determined.

GenotypeSeed FAMEEndosperm FAMEHypocotyl Length (mm) − SucHypocotyl Length (mm) + Suc
μg/seedμg/seed
Wild Type5.1 ± 0.80.72 ± 0.318.2 ± 2.119.4 ± 2.2
Line 1014.4 ± 0.2n.d.14.0 ± 0.317.6 ± 2.8
Line 1066.4 ± 1.11.1 ± 0.516.6 ± 2.17.1 ± 6.2
wri1-11.4 ± 0.40.2 ± 0.16.7 ± 0.710.6 ± 4.8
Wild type × wri1-1n.d.0.7 ± 0.1n.d.n.d.
wri1-1 × wild typen.d.0.7 ± 0.2n.d.n.d.