Table II.

Purification of TLG1 from L. edodes fruiting bodies at day 4 after harvest

Glucanase activity was measured by the method of Somogyi-Nelson using CM Pachyman as a substrate.

Total ProteinTotal ActivitySpecific ActivityPurificationYield
mgunitunit/mg
Extract from fruiting body9.148416.545.531
50% ammonium sulfate precipitate2.2228.3612.7710.24
Superdex 75 10/300.29616.4455.544.350.03
Mono Q 5/50 GL0.0060.6911590.01