Table I.

Arabidopsis proteins whose abundance was significantly different in wild-type germinating Ler seeds in the presence or absence of 0.5 mm of SA 24 h post imbibition

DS, Dry seeds; H2O, seeds imbibed for 24 h on water; SA, seeds imbibed for 24 h in the presence of 0.5 mm of SA; % Cov., coverage; Exp., experimental; MM, molecular mass; Th., theoretical; AGI, Arabidopsis Genome Initiative; H2O/DS, normalized spot volume in the Arabidopsis seeds incubated for 24 h in water divided by the normalized spot volume in the dry mature seeds, from three different gels and independent extractions; SA/DS, normalized spot volume in the Arabidopsis seeds incubated for 24 h in the presence of 0.5 mm SA divided by the normalized spot volume in the dry mature seeds, from three different gels and independent extractions; SA/H2O, normalized spot volume in the Arabidopsis seeds incubated for 24 h in the presence of 0.5 mm SA divided by the normalized spot volume in the Arabidopsis seeds incubated for 24 h in water, from three different gels and independent extractions.

No.Exp. MMExp. pIArabidopsis Protein Name% Cov.Th. MMTh. pIAGI No.Ratioa H2O/DSRatioa SA/DSRatioa SA/H2O
kDkD
70b49.437.1912S seed storage protein precursor22%52.497.68At5g441200.960.310.32
71b50.447.6712S seed storage protein precursor34%52.497.68At5g441200.670.180.27
329b50.447.6712S Seed storage protein precursor35%52.497.68At5g441203.981.170.29
504c60.876.2012S seed storage protein precursor7%55.866.36At4g285202.151.080.50
505c28.875.3020S proteasome α subunit A1 (PAA1)23%27.285.66At5g355902.981.010.34
510c25.984.0020S proteasome α subunit E2 or E1 (PAE2 or PAE1)9%25.964.41At3g14290 or At1g53850≤0.0011.22≥1,000
520c32.815.99α-Cruciferin 12S (seed storage protein fragment)12%31.639.08At5g4412045.212.020.04
159b23.545.33α-Cruciferin 12S (seed storage protein fragment)15%27.246.34At4g285201.012.662.63
76b23.945.58α-Cruciferin 12S (seed storage protein fragment)16%34.686.42At4g285200.852.062.42
521c25.345.81α-Cruciferin 12S (seed storage protein fragment)40%34.686.42At5g441201.002.92.90
518c15.828.66α-Cruciferin 12S (seed storage protein fragment)36%20.849.06At5g441201.0031.9531.95
519c15.168.80β-Cruciferin 12S (seed storage protein fragment)53%20.849.06At5g441201.0027.7927.79
5b57.354.89Tubulin-α 3.527%50.734.70At5g19770≥1,000≥1,0001.51
33b14.865.87β-Cruciferin 12S (seed storage protein fragment)18%20.849.06At5g441200.962.712.82
420c15.054.81Cytochrome b549%15.074.93At5g535601.00≥1,000≥1,000
254b22.104.96Dehydrin6%18.467.10At5g664000.321.163.63
255b21.655.18Dehydrin6%18.467.10At5g664000.291.214.17
11b19.615.75EM-like protein3%16.615.75At3g518100.591.091.85
361b47.755.224-Methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis protein6%41.845.08At3g149902.183.231.48
365b62.776.30Isocitrate lyase16%50.426.29At3g21720240.34634.522.64
167b62.968.00Malate synthase25%63.898.02At5g038601.283.542.77
168b62.917.08Malate synthase10%63.898.02At5g038601.462.171.49
509c34.114.01NAC domain-containing protein24%17.874.85At5g1385064.94183.042.82
513c18.675.50NAC domain-containing protein6%17.996.00At1g7323051.15102.312.00
516c16.116.30Pathogenesis-related protein23%19.126.34At1g708400.181.8910.50
515c23.945.89Superoxide dismutase21%25.448.83At3g109200.441.272.89
523c23.946.42Superoxide dismutase16%26.876.76At3g563504.4818.934.23
512c19.415.50Temperature stress induced lipocalin12%21.426.29At5g580703.137.382.36
  • a Data obtained from densitometric analysis of individual spots from proteins in 2D gels stained with silver nitrate.

  • b Listed proteins correspond to previously identified proteins (Gallardo et al., 2001, 2002a; Rajjou et al., 2004; Job et al., 2005).

  • c Listed proteins correspond to proteins identified during this work; the peptide sequences determined are available in Supplemental Table V.