Table I.

Predicted NLS in ICK1/KRP1 and here generated mutant variants

[KR], K or R; [KR]{2,3}, between 2 and 3 Ks or Rs; x, any amino acid. PredictNLS server is at http://cubic.bioc.columbia.edu/predictNLS/. Amino acid sequence present in ICK1/KRP1 that shows similarity to the predicted NLS sequence is given in bold face. Amino acid residues that have been mutated to interfere with the NLS are underlined.

ObjectAmino Acid Sequence
Consensus 1[KR]{2,3}xxKR[KR][QLM]
Consensus 2RRxxKRK
ICK1/KRP1 wild type75-ETSTYRRGTKRKLFENLREEE-95
R80/81A75-ETSTYAAGTKRKLFENLREEE-95
K84/86A75-ETSTYRRGTARALFENLREEE-95