Table I.

Purification of cytosolic DGAT from developing peanut cotyledons

The results are the summary of a typical purification of DGAT. Frozen immature peanut cotyledons (100 g) were used for preparing the cytosol. Enzyme activity measurements and the purification steps are described in “Materials and Methods.”

FractionProteinTotal ActivitySpecific ActivityPurification
mgnmol min1pmol min1 mg1-fold
Cytosol1,6046.584.11
Octyl-Sepharose19.424.93253.762
Blue-Sepharose1.392.371,707.4416
Superdex 750.471.453,073.8750
Heparin-agarose0.161.137,069.51,724