Expression analysis by quantitative RT-PCR of 10 LysM-RLK genes of M. truncatula
mRNA levels were normalized against ACTIN2 and values were calculated as ratios relative to root expression levels. The averages of two independent biological experiments are presented. Root samples always have a value of 1 and sems are given for other samples. Bold text indicates preferential localization of gene expression. ND, Not determined.
| Gene | Expression Levels Relative to Roots | Root Expression Relative to NFP | ||||||
|---|---|---|---|---|---|---|---|---|
| Root | Nodule | Leaf | Stem | |||||
| NFP | 1 | 0.59 ± 0 | 0 | 0.024 ± 0.02 | 1 | |||
| LYR1 | 1 | 0 | 0 | 0 | ND | |||
| LYR2 | 1 | 1.75 ± 0.035 | 0 | 0 | 0.37 | |||
| LYR3 | 1 | 2.90 ± 0.89 | 0.23 ± 0.11 | 0.005 ± 0.004 | 6.23 | |||
| LYR4 | 1 | 2.27 ± 0.59 | 10.50 ± 5.78 | 1.70 ± 0.95 | 21.56 | |||
| LYR5 | 1 | 6.39 ± 0.54 | 21.79 ± 0.24 | 0.26 ± 0.02 | 2.43 | |||
| LYR6 | 1 | 3.80 ± 3.95 | 0.26 ± 0.032 | 0.008 ± 0.004 | 12 | |||
| LYK8 | 1 | 0.06 ± 0 | 0.01 ± 0.007 | 0 | 1.22 | |||
| LYK9 | 1 | 0.74 ± 0.12 | 2.33 ± 0.76 | 0.28 ± 0.085 | 38.32 | |||
| LYK10 | 1 | 0.73 ± 0.10 | 0 | 0 | 0.15 | |||