Table I.

Estimated off-target patterns during PTGS in plants

SpeciesSource Sequence SetRelease DateTotal No. Unique Gene TranscriptsNo. Triggers Predicted to Silence Off-Targets (% Total)Average No. Predicted Off-Targets/TriggerAverage No. Predicted Off-Target Regions/Off-TargetAverage Length (nt)/Off-Target RegionAverage No. Efficient siRNAs/Trigger
ArabidopsisATH16/10/0428,95219,882 (68.7)3.92.941.235.2
ArabidopsisAGI 111/29/0445,68337,250 (81.5)4.3399.2147.5
Capsicum annuumCaGI 16/4/0410,71227,88 (26.0)1.92.240.712.5
Gossypium spp.CGI 59/16/0324,35014,270 (58.6)6.92.37487.5
Chlamydomonas reinhardtiiChrGI 41/31/0430,33916,579 (54.6)6.11.940.212.5
Glycine maxGmGI 119/16/0367,82649,658 (73.2)9.42.177.9108.4
Helianthus annuusHaGI 39/16/0320,52011,961 (58.3)15.81.434.638.8
Hordeum vulgareHvGI 81/30/0449,19032,297 (65.6)16.32.699.4205.9
TomatoLeGI 95/19/0331,01215,340 (49.5)2.42.360.830.9
Lotus japonicusLjGI 25/16/0311,0252,951 (26.8)2.61.735.610
LettuceLsGI 22/3/0422,18512,345 (55.6)24.11.430.426.8
Mesembryanthemum crystallinumMcGI 45/15/038,4553,459 (42)13.323329.4
Medicago truncatulaMtGI 75/19/0336,97619,468 (52.7)5.62.249.450.6
Nicotiana benthamianaNbGI 11/28/046,11831,99 (52.3)2.83.853.442.1
Nicotiana tabacumNtGI 16/4/0410,2322,184 (21.3)1.83.138.315.2
Allium cepaOnGI 19/17/0311,7263,735 (31.9)1.72.354.220.2
RiceOsGI 155/27/0488,76545,406 (51.2)22.54.441.7174.3
Pinus spp.PGI 41/28/0431,77116,487 (51.9)11.75.538.427.5
RyeRyeGI 31/29/045,3471,472 (27.5)2.7632.410.7
Sorghum bicolorSbGI 85/26/0439,14822,039 (56.3)6.41.970.356.9
SugarcaneSoGI 11/30/0495,88455,269 (57.6)24.13.569.1205.4
Solanum tuberosumStGI 95/26/0432,55320,469 (62.9)4.42.946.242.2
Triticum aestivumTaGI 81/29/0412,380789,862 (72.6)19.52.752.4102.2
Theobroma cacaoTcaGI 16/4/042,539531 (20.9)2.61.937.413
Vitis viniferaVvGI 311/14/0323,10910,548 (45.6)8.81.840.337.1
Zea maysZmGI 141/2/0456,36440,028 (71)8.9363.196.9