Result from the siRNA Scan search for potential off-targets with the cloned sequences of N. benthamiana TC381 and TC1146 (Supplemental Fig. S1) as trigger sequences
TC numbers in bold are the ones used for quantitative RT-PCR.
| Target ID | Annotation of the Target | Potential Off-Target | Identical (I) or Reverse Complementary (RC) Sequence | Annotation of the Potential Off-Target |
|---|---|---|---|---|
| TC381 | Similar to TIGR_Osa1|9630.m01295 U2 snRNAP protein, Arabidopsis, partial 79% | CK286172 | RC, GGGTGTATTCTGGCCCGGGCC TGRC, TATCTTGTATAGTAGTTATTAGTATAGT | Similar to UP|O93419 (O93419) collagen XVIII precursor, partial (1%). |
| CK288691 | RC, TATCTTGTATAGTAGTTATTAGTATAGT | Annotation not available. | ||
| CK289650 | I, TTCGCGGTCCCGGGCTTCGTG | Similar to TIGR_Osa1|9631.m00421 CYFIP2, partial (9%). | ||
| TC10748 | I, TATCTTGTATAGTAGTTATTA GTATA | Similar to TIGR_Ath1|At5g13210.1 68418.m01516 expressed protein, partial (17%). | ||
| TC7796 | I, TATCTTGTATAGTAGTTATTA TGTATAG | Weakly similar to UP|Q75NB3 (Q75NB3) Cys proteinase, partial (34%). | ||
| TC1146 | Similar to UP|Q8H9C6 (Q8H9C6) pyruvate decarboxylase (fragment), partial (33%) | CK282591 | RC, ATCGCTTTGCGAACCCGACTAG | Similar to UP|Q6Z3U3 (Q6Z3U3) VP1/ABI3 family regulatory protein-like, partial (5%). |
| CK287535 | RC, ATCGCTTTGCGAACCCGACTAG | Similar to TIGR_Ath1|At5g65980.1 68418.m08307 auxin efflux carrier family protein contains auxin efflux carrier domain, Pfam:PF03547, partial (14%). | ||
| CK292351 | RC, TTTGTTCTCGGGCCTTTACCAG | Similar to UP|CP22_HORVU (P55748) Ser carboxypeptidase II-2 precursor (CP-MII.2) (fragment), partial (30%). | ||
| CK296810 | RC, ATCGCTTTGCGAACCCGACTAG | Similar to TIGR_Ath1|At5g65980.1 68418.m08307 auxin efflux carrier family protein contains auxin efflux carrier domain, Pfam:PF03547, partial (11%). | ||
| TC8666 | RC, ATCGCTTTGCGAACCCGACTAG | Similar to TIGR_Ath1|At2g17500.1 68415.m02022 auxin efflux carrier family protein contains auxin efflux carrier domain, Pfam:PF03547, partial (41%). |