Table I.

Protein spots identified with MALDI-TOF and/or LC-MS/MS

Mass spectrometric identification of protein spots with differential accumulation after OS elicitation using comparison of the measured sequences or peptide mass fingerprints with the NCBI green plant protein database. Type of response, as indicated by Roman numerals, is described in the text. No. Pep., Number of peptides.

Spot No.Accession No.Name of ProteinProtein Identification Data
MALDI (Score/No. Pep.)LC-MS/MS(Score/No. Pep.)Theoretical kD/pIObserved kD/pIPeptide Sequence (MS/MS)Response
1XP_472335OSJNBa0039C07.4 protein9.7/1812.71/198.4/5.9887.2/5.79(K)TAIAEGLAQR(I)II
2S58083TK9.79/1212.07/675/672.3/7.05(R)FLAIDAVEK(A)III
(K)NGNTGYDEIR(A)
(K)VTTTIGFGSPNK(A)
(K)ANSYSVHGSGLGAK(E)
(K)ALPTYTPESPADATR(N)
(R)KTPSILALSR(Q)
4BAA77604Plastidic ALD5.69/412.77/342.5/7.4835.4/7.23(K)YTGEGESEEAK(K)No change
(R)SAAYYQQGAR(F)
(K)YTGEGESEEAKK(G)
5AAO23981Vacuolar H+-ATPase A2 subunit8.71/1110.28/268.6/5.3768.7/5.54(R)NLEDETR(-)I
(R)SGDVYIPR(G)
6Q40565RCA10.13/1211.62/548.3/8.1740.3/5.02(R)TDNVPEEAVIK(I)II
(R)VYDDEVRK(W)
(K)WVSGTGIEAIGDK(L)
(K)LLNSFDGPPTFEQPK(M)
(R)VQLAETYLK(E)
7AAG59585TD12.43/1012.72/665.6/6.2164.5/5.94(R)VYDVAIDSPLQNAAK(L)I
(R)EDMQSVFSFK(L)
(K)DVYDEGR(N)
(K)QALVLYSVGVNTK(S)
(K)SSQLNTVNLTNNNLVK(E)
(K)FLDAFSPR(W)
8AAM62737QR-like protein12.77/132.7/6.0863/5.56(K)VVAAGLNPVDAKR(R)III
AAB39828Chaperonin11.51/118.10/10.2(K)IVNDGVTVAR(E)
9Q40565Rubisco activase11.62/1148.3/8.1739.1/5.25(K)GLVQDFSDDQQDIAR(G)II
(R)QYNMDNTLDGFYIAPSFMDK(L)
(K)VPLILGMWGGK(G)
(R)KMGINPIMMSAGELESGNAGEPAK(L)
(K)MGINPIMMSAGELESGNAGEPAK(L)
(R)VYDDEVR(K)
(R)KWMSGTGIEAIGDK(L)
(K)LLNSFDGPPTFEQPK(M)
(K)LLEYGNMLVQEQENVKR(V)
(K)RVQLAETYLK(E)
(K)EAALGDANADAINTGNF(-)
122002393AOxygen-evolving complex protein5.17/411.46/326.48/4929.8/4.83(R)RLTFDEIQSK(T)II
(K)FCLEPTSFTVK(A)
(R)GGSTGYDNAVALPAGGR(G)
13AAL71857DHAR4.07/423.6/8.222.8/8.3I
14BAB64833PGM6.02/710.4/261/6.363.7/6.17(K)LVDLALASGK(I)III
(R)LDQVQLLLK(G)
16AAP04394Glutathione S-transferase12.69/11/4.0723.6/8.37(-)LLDVYETR(-)I
17Q42961Phosphoglycerate kinase precursor5.2/1250.1/8.7541.6/6.99V
18NP_199203Myosin heavy-chain MYA21.9/4169.8/7.920.5/8.08I
19BAA77604Plastidic ALD5.71/542.5/7.435.9/6.37V
Unspecified12.36/11.4/6.93(-)GAVQLWKYLEDR(-)
20Q40565Rubisco activase8.95/1614.54/748.3/8.437.8/7.19(K)LVVHITK(N)I
(K)SFQCELVFR(K)
(R)EAAEIIR(K)
(R)EAAEIIRK(G)
(R)TDNVPEEAVIK(I)
(R)VYDDEVRK(W)
(R)VQLAETYLK(E)
21AAT38067Putative glycoprotease1.2/440.8/6.1945.1/<4.55I
Unspecified12.72/10.9/11.7(-)VVKKKGFR(-)
22AAS903243-Dehydroquinate dehydratase5.31/456.1/6.0832.4/7.93I
Unspecified12.72/11.1/9.71(-)NAGANAYAALTK(-)
23BAD28273Hypothetical protein5.38/422.2/9.735.3/>9.3IV
24AAM97013Unknown protein Arabidopsis7.37/5113.5/9.0626.6/4.67I
BAD32968RNA-binding protein-like12.77/135.6/10.4(R)YADPVQR(E)
25Q9M5M660S ribosomal protein L303.62/412.2/9.687.2/8.48IV
Putative subtilase11.6/11.2/9.71(-)TVNTVGAGAATYK(-)
27Unspecified12.72/11.5/8.8923.9/4.8(-)LKPDPNTCGGKDFK(-)IV
28S72477Probable cinnamyl-alcohol dehydrogenase12.72/138.3/5.8638.9/8.51(R)DTSGVLSPFNFSR(R)I
29AAB33843Aldehyde dehydrogenase4.95/452.6/5.6455/7.14I
30CAA79702Mitochondrial formate dehydrogenase precursor8.54/711.59/641.8/7.0837/8.5(K)IVGVFYK(A)I
(K)TVGTVGAGR(I)
(K)MDSELENQIGAK(F)
(K)FEEDLDK(M)
(K)GVLIVNNAR(G)
(K)DGELAPQYR(-)
32AAM28874ACS74.53/477.2/6.5135.3/8.89IV
34P43282S-adenosylmethionine synthetase8.4/411.38 /242.6/6.0746.3/7.14(K)SVVASGLAR(R)I
(K)TQVTVEYK(N)
35CAF22093Glyceraldehyde-3-P dehydrogenase12.21/219.59/8.8235/8.84(R)AASFNIIPSSTGAAK(A)II
(K)VVISAPSK(D)
37BAD52936Putative homocysteine S methyltransferase 44.73/534.5/6.0917.9/8.85IV
Unspecified12.72/11.2/6.75(-)QLMDAATTTNAK(-)
38AAM65239Unknown protein5.74/432.3/10.132.6/7.96V
1SM4AFerredoxin Nadp reductase11.22/233.3/6(R)LYSIASSAIGDFGDSK(T)
(K)DNTFVYMCGLK(G)
39NP_054979Hypothetical protein SpolCp0752.32/47/10.835.3/>9.3I
40AAF27063F4N2.234.38/495.3/6.2237.6/5.68V
42AAM46780Latex plastidic ALD6.51/442.6/8.635.1/5.91V
43BAA01279O-acetylserine thiol lyase3.89/434.1/5.6119.6/8.08I
45CAA75381Translation elongation factor5.34/1013.28/127.2/5.0942/4.64(R)NTTVTGVEMFQK(I)I
46Q40565Rubisco activase7.82/1312.72/1334.36/6.7635.1/6.14(K)IVVHITK(N)I
(K)SFQCELVFR(K)
(K)SFQCELVFRK(M)
(R)YREAAEIIR(K)
(R)EAAEIIRK(G)
(K)FYWAPTR(E)
(R)VYDDEVR(K)
(R)VYDDEVRK(W)
(K)WVSGTGIEAIGDK(L)
(K)LLNSFDGPPTFEQPK(M)
(K)LLEYGNMLVQEQENVK(R)
(K)LLEYGNMLVQEQENVKR(V)
(R)VQLAETYLK(E)
47P49319Catalase isozyme 1 salicylic acid-binding protein5.15/1856.7/7.0254.9/8.64V
48AAD39604F23M19.32.69/467/9.573.2/9.09V
49CAD27635Maturase4.22/461.2/9.6824.5/>9.3IV
50BAA24535Spermidine synthase5.81/8−13.9/134.4/5.335/4.55(K)ASFCLPSFAK(R)I
51CAA78703Rubisco activase8.99/814.54/648.3/8.433/6.16(K)SFQCELVFR(K)I
(R)TDNVPEEAVIK(I)
(R)VYDDEVR(K)
(K)LLNSFDGPPTFEQPK(M)
(R)VQLAETYLK(E)
(K)EAALGDANADAINTGNF(-)
53CAE05740OSJNBb0017I01.201.68/440.8/5.724.5/>9.3IV
54AAG40343Hydroxymethyltransferase5.94/451.7/7.655/8.73IV
55NP_918618OSJNBa0094H06.182.33/45.6/10.122/>9.3IV
57AAF97728Potassium-sodium symporter2.52/461.9/9.1338.9/7.53I
58NP_915483P0446B05.255.04/438.6/9.642.1/8.5I
60XP_470450Unknown protein3.86/448.2/10.537/7.37I
61T07032S-2-hydroxy acid oxidase6.05/731.2/9.5744.5/>9.3I
62XP_468473Putative cytochrome P4505.2/458.1/9.441.7/5.89V
64BAD03386Hypothetical protein3.33/420.8/10107.3/<4.5IV
65CAA96433PME5.1/411.46/120.1/9.2937/8.31(R)VGADMSVINR(C)IV
66CAC80376Glyceraldehyde-3-P dehydrogenase5.81/412.71/534/5.5636.2/8.33(K)TLLFGEK(S)II
(K)KVVISAPSK(D)
(R)AASFNIIPSSTGAAK(A)
(K)EASYDDIK(A)
(K)AAIKEESEGK(L)
67AAN40027Hypothetical protein3.48/564.7/9.0937.9/<4.5V
68P29790ATP synthase γ-subunit9.47/741.4/8.435/8.64IV
CAB39974Glyceraldehyde-3-P dehydrogenase12.72/534.4/6.15(K)DAPMFVVGVNEK(E)
(R)AASFNIIPSSTGAAK(A)
(K)VLPSLNGK(L)
(K)EATYDEIK(A)
(K)AAIKEESEGK(L)
69AAF03099NADP-dependent glyceraldehyde phosphate dehydrogenase11.71/111.8/10.831/7.93(K)DSPLDVIAINDTGGVK(Q)IV
70AAG23799Glyceraldehyde-3-P dehydrogenase12.66/318.2/6.135.1/8.73(R)AAALNIVPTSTGAAK(A)V
(K)AVALVLPSLK(G)
(K)TFAEEVNAAFR(E)
71AAM62933Putative chloroplast 50S ribosomal protein L614.54/424.6/10.217.4/>9.3(R)TLTDNMVVGVSK(G)V
(R)ITVSGYDK(S)
(K)SEIGQFAATVR(K)
(K)YADEIVR(R)
722119042Gly-rich RNA-binding protein10.74/914.54/315.6/5.6215/5.11(R)NITVNEAQSR(G)II
(R)EGGYGGGGGYGGGR(R)
(R)EGGYGGGGGGGYGGGR(R)
74P81665Oxygen-evolving enhancer protein 111.12/912.72/226.39/4.3130.2/<4.55(R)GSSFLDPK(G)II
(R)GGSTGYDNAVALPAGGR(G)
75P81665_1Oxygen-evolving enhancer protein12.49/11.09/4.0730.2/<4.55(K)NAPPEFQK(T)II
77T45856Hypothetical protein F3A4.1104.26/457.2/9.4630/<4.55V
AAP03871Oxygen-evolving complex 33-kD PSII protein12.71/635.17/5.4(K)RLTFDEIQSK(T)
(R)LTFDEIQSK(T)
(K)FCLEPTSFTVK(A)
(K)DGIDYAAVTVQLPGGER(V)
(R)GGSTGYDNAVALPAGGR(G)
(R)GDEEELEKENVK(N)
79B24430Glyceraldehyde-3-P dehydrogenase6.51/810.85/441.78/8.5641.6/8.45(K)YDSMLGTFK(A)V
(K)IVDNETISVDGK(H)
(K)GTMTTTHSYTGDQR(L)
(R)AAALNIVPTSTGAAK(A)
80AAR960064-Nitrophenylphosphatase-like protein11.68/116.5/5.3829.6/5.18(R)LVFVTNNSTK(S)V
821909374ARubisco activase7.01/1142.7/5.5741/5.59II
83AAO62992Chloroplast Gln synthetase12.73/220.9/5.8241.7/5.89(K)AAQIFSDSK(V)V
(R)DISDAHYK(A)
84AAA34111Rubisco7.71/410.1/5.3614.8/6.37V
85AAG14029NADH dehydrogenase3.86/437.4/9.6927/>9.3II
87BAA31510Ribosomal protein L4 chloroplast2.85/431.2/6.5927.6/8.71II
89BAD35631Putative zinc-finger and C2 domain protein3.56/435.6/6.4730.9/>9.3II
90AAM64724Unknown protein3.22/461.7/5.2387.9/<4.5V
91BAD15634Mitochondrial transcription termination factor-like3.65/442.6/9.935.9/6.37V
92CAB87677Putative protein4.78/534.7/6.5642.2/<4.5II
93AAR20278Maturase6.32/555.1/10.442.9/4.55V
94AAO32311Putative chloroplast 50S ribosomal protein L65.29/624.9/10.318.9/>9.3III
95CAA78703Rubisco activase9.14/1211.62/648.3/8.441.3/4.71(K)GLVQDFSDDQQDIAR(G)II
(R)EAAEIIR(K)
(R)TDNVPEEAVIK(I)
(R)VYDDEVR(K)
(K)WVSGTGIEAIGDK(L)
(R)VQLAETYLK(E)
97NP_174644Hypothetical protein2.74/421.6/7.719.7/<4.5V
98BAD28931Hypothetical protein2.39/57.4/10.339.7/8.6V
99AAF76363I box-binding factor2.77/423.9/8.3142.7/>9.3V
100BAD53039Hypothetical protein2.88/47.3/6.4741/8.73V
101XP_466540Putative speckle-type POZ4.05/838.8/10.576.2/5.25V
103P29302PSI chain II D2 precursor5.71/522.4/10.141.5/9III
105CAA71589Protein g5bf3.82/442.6/3.8237/8.48III
108AAU03361PS2 oxygen-evolving complex protein 314.51/10.95/10.915/>9.3(-)GKLKGSGKGK(-)II
109O23787Thiazole biosynthetic enzyme precursor9.53/1111.62/137.5/5.232.9/4.8(K)ALDMNSAEDAIVR(L)IV