Table II.

Changes of proteins in N. attenuata leaves upon several induction treatments

The accumulations of N. attenuata leaf proteins exhibiting consistent up- or down-regulation (indicated by arrows) at a minimum of three time points (6–72 h) after OS treatment are listed with their P values of water control (W + W), OS (W + OS), FACs (W + FAC) induction, OS without FACs (W + OS-FAC-free), and caterpillar-feeding induction (Larvae) at 48 h. The protein names represent the database identification of the mass spectrometric analyses with the highest confidence. Numbers indicate P values; n/a, P value is not available; –, no change; and x, spot not detectable. qltv, Qualitative change in protein spots.

Spot No.Accession No.Name of ProteinInduction Pattern and P Values for the Treatments
W + WW + OSW + FACW + OS-FAC-FreeLarvae
Photosynthesis and photorespiration-related metabolism
6CAA78703Rubisco activase↓ 0.19↓ 0.93↓ 0.11↓ 0.07
9XP_473501, Q40565OSJNBb0078D11.3, Rubisco activase↓ 0.04↓ 0.21↓ 0.003↓ 0.05
12Q40459Oxygen-evolving enhancer protein 1↓ 0.31↓ 0.45
20CAA78703Rubisco activase↑ qltv↑ 0.18
46CAA78703Rubisco activase↑ 0.01↑ qltv↑ 0.05↑ 0.15↑ 0.07
51CAA78703Rubisco activase↑ 0.02↑ qltv↑ 0.18↑ 0.19
74P81665Oxygen-evolving enhancer protein 1↓ 0.05↓ 0.11↑ 0.06
75P81665_1Oxygen-evolving enhancer protein 1↓ 0.11↓ 0.15↓ 0.62
77AAP03871Oxygen-evolving complex 33-kD PSII protein↓ 0.05↓ 0.12
821909374ARubisco activase↓ 0.27↓ 0.58↓ 0.02↓ 0.12
95CAA78703Rubisco activase↑ 0.06↓ 0.27↓ 0.41↓ 0.02
108AAU03361Oxygen-evolving complex protein 3↓ 0.01
Primary metabolism
1XP_472335OSJNBa0039C07.4↑ 0.09↑ 0.15
2S58083TK↑ 0.04↑ 0.77↑ 0.18
5AAO23981Vacuolar H+-ATPase A2 subunit↑ 0.13↑ 0.08↑ 0.02
7AAG59585TD↑ 0.17↑ 0.24↑ 0.34↑ 0.02
21AAT38067Putative glycoprotease↑ qltv
29AAB33843Aldehyde dehydrogenase↑ 0.12↑ 0.28x n/a
30CAA79702Mitochondrial formate dehydrogenase precursor↑ 0.35↑ 0.18
32AAM28874ACS7↑ 0.22↑ 0.27↑ n/a↑ n/a
34AAO85809S-adenosylmethionine synthetase↑ 0.25↑ 0.06↑ 0.11↑ 0.03
35CAF22093Glyceraldehyde-3-P dehydrogenase↓ 0.8- n/a↑ 0.004
43BAA01279O-acetylserine thiol lyase↑ qltv↑ 0.11↑ 0.23↑ 0.18
50BAA24535Spermidine synthase↑ 0.09↑ 0.08↑ 0.01↑ n/a
66BAC87864Glyceraldehyde-3-P dehydrogenase↓ 0.26↓ 0.08
83AAO62992Chloroplast Gln synthetase↑ 0.03
Transcription and translation
45BAA02028Translation elongation factor↑ qltv
72BAA03741Gly-rich RNA-binding protein↓ 0.02↓ 0.03- 0.51
87BAA31510Ribosomal protein L4 chloroplast↓ qltv- n/a- n/a
Secretory pathway
57AAF97728Potassium-sodium symporter↑ 0.08↑ 0.11↑ 0.04↑ 0.008- n/a
Secondary metabolism
8AAM62737QR-like protein↓ 0.37↓ 0.005↓ 0.21
22AAS903243-dehydroquinate dehydratase↑ 0.21↑ 0.002↑ 0.77
24BAD32968RNA-binding-like protein↑ 0.18↑ 0.05↑ 0.02↑ 0.04↑ 0.003
Unspecified function and unknown proteins
58NP_915483P0446B05.25↑ 0.08↑ 0.25
92CAB87677Putative protein↓ 0.04