Table I.

Endogenous BR levels (as determined by GC-MS-selected ion monitoring) in wild-type plants and BR-deficient and -response mutants

For experiment 1, the tissue harvested included the apical bud and the three internodes below, without any expanded leaf tissue from 26-d-old seedlings. For experiments 2 and 3, the tissue harvested consisted of the whole shoot from 14-d-old and 25-d-old seedlings, respectively. Data are shown as means ± se (n = 2). nd, Not detected; NA, not analyzed.

Level
Wild-Typelkelkblka
ng/g fresh weight
Experiment 1
    6-DeoxoCS3.70 ± 0.485.25 ± 0.170.21 ± 0.04NA
    CS0.44 ± 0.010.31 ± 0.050.09 ± 0.01NA
    BLndndndNA
Experiment 2
    6-DeoxoCS2.39 ± 0.063.64 ± 0.100.09 ± 0.014.50 ± 0.29
    CS0.56 ± 0.070.26 ± 0.000.27 ± 0.071.80 ± 0.29
    BLndndndnd
Experiment 3
    6-DeoxoCS3.50 ± 0.604.22 ± 0.03NANA
    CS0.17 ± 0.010.10 ± 0.01NANA
    BLndndNANA