Table II.

Composition of rapeseed MDE cultures used for proteome analysis

The percentage of embryo formation relative to the number of microspores at the start of culture was determined in subsamples of the starting cultures grown continuously for 10 d at 32°C. The 10-d embryo cultures (10e) were sieved as described in Table I to obtain pure populations of embryos. The number of spots detected in each of the 2-D gels was determined by PDQuest software. 0d, Freshly isolated microspores; 5p, 5-d pollen culture; 2e, 2-d conventional MDE culture; 5e, 5-d conventional MDE culture; 10e, 10-d conventional MDE culture; 8se, 8-d suspensor-bearing MDE culture; 10se, 10-d suspensor-bearing MDE culture.

Stage% Embryo (Pool 1/Pool 2)% Embryo (Average)No. Spots (Gel 1/Gel 2)Average
0d576/479528
5p1,239/8831,061
2e7/45.5722/673698
5e5/55639/538589
10e2/431,007/908958
8se2/53.5884/1,053969
10se2/641,046/1,2731,160