Table III.

Differentially expressed proteins identified in rapeseed MDE cultures

Each entry contains a number that corresponds to the protein spots indicated in Figure 4. Matched Peptides gives the number of peptide sequences identified by LC-MS/MS that showed homology to the best-matching gene product. The molecular mass and isoelectric point (IEP) as estimated from the 2-D gel are given, and in parentheses the deduced molecular mass and IEP of the best-matching gene product. The last column represents the percentage of sequence coverage by the matched peptides to the most similar protein in the database. At, Arabidopsis; Bj, Brassica juncea; Bn, rapeseed; Bo, Brassica oleracea; Br, Brassica rapa.

No.Best-Matching Gene Product (Organism)IDProcessMatched Peptides (n)Molecular Mass Hit (kD)IEP Hit (pI)Coverage (%)
1Expressed protein (At)At5g48480Unknown320.1 (17.6)4.60 (4.80)16.8
2Proliferating cellular nuclear antigen (At)At2g29570DNA replication535.7 (29.2)4.49 (4.39)36.7
Proliferating cellular nuclear antigen (Bn)gi15222379DNA replication535.7 (29.2)4.49 (4.42)36.7
314-3-3 protein (Bn)gi13447112Signaling533.5 (18.2)4.64 (5.19)20.8
4Disulfide isomerase-like protein (At)At1g21750Redox processes1461.3 (55.6)4.66 (4.61)25.9
5CCAAT-box binding factor Hap5a subunit (At)At1g08970Transcription431.2 (25.6)5.02 (4.91)16.4
6Rubisco subunit binding protein (Bn)gi464727Protein modification/processing862.7 (61.6)4.81 (4.95)18.7
7P-ribosylformylglycinamidine synthase (At)At1g74260Secondary metabolism2126.1 (151.7)4.97 (4.99)2.1
8Thiazole biosynthetic enzyme precursor (At)At5g54770Secondary metabolism334.6 (36.6)5.06 (5.78)9.7
9Monodehydroascorbate reductase (At)At5g03630Redox processes249.3 (47.5)5.11 (5.06)6.7
10a40S ribosomal protein (Bn)gi15214300Protein synthesis342.3 (32.0)5.12 (4.94)13.7
10bGln synthetase (Bn)gi1084350Amino acid metabolism342.3 (39.1)5.12 (5.13)9.5
11H+-transporting two-sector ATPase (At)At3g28710Electron transport342.4 (40.7)5.16 (4.81)12.8
12HSP90 heat shock protein (At)At2g04030Protein modification/processing297.7 (88.6)5.17 (4.74)3.5
13GST (At)gi15218518Redox processes226.5 (18.8)5.39 (5.64)13.5
14Chloroplast Cpn21 protein (At)At5g20720Protein modification/processing528.7 (26.8)5.46 (9.35)21.3
15Lactoylglutathione lyase/glyoxylase I (Bo)gi2494843Redox processes1134.2 (31.6)5.31 (4.97)24.8
16Dehydroascorbate reductase (Bj)gi22653413Redox processes330.7 (28.5)5.47 (8.40)11.7
Dehydroascorbate reductase (Bj)gi22653415Redox processes330.7 (24.3)5.47 (5.19)13.8
17Phoshoglycerate kinase (At)At1g56190Carbohydrate metabolism744.2 (49.9)5.32 (6.23)22.4
18Peroxiredoxin (Br)gi4928472Redox processes621.5 (17.4)5.48 (5.23)41.3
19Expressed protein (At)At5g02240Unknown1033.9 (27.1)5.57 (6.20)19.3
20Phosphoglycerate kinase (At)At1g79550Carbohydrate metabolism1042.5 (42.1)5.48 (5.33)23.2
21Chaperonin (At)At3g13860Protein modification/processing1360.5 (60.4)5.54 (5.74)13.8
22Chaperonin; T-complex protein (At)At3g03960Protein modification/processing260.8 (58.9)5.63 (5.07)2.7
23Dehydroascorbate reductase (Br)gi33285914Redox processes429.6 (12.0)5.86 (6.16)33.3
24Expressed protein (At)At5g02240Unknown632.9 (27.1)5.87 (6.20)6.3
25l-Ascorbate peroxidase (Bn)gi1890354Redox processes1330.9 (27.7)5.96 (5.83)42.4
26Glutamate decarboxylase (At)At2g02010Signaling457.3 (55.9)6.09 (6.26)6.4
27aLectin (At)At3g16400Carbohydrate binding265.5 (51.6)5.81 (5.18)5.6
27b2,3-Biphosphoglycerate-independent phosphoglycerate mutase (At)At3g08590Carbohydrate metabolism365.5 (60.7)5.81 (5.43)6.3
28GST (GST6; At)At2g47730Redox processes328.3 (19.7)6.30 (5.22)26.0
29GST (Bj)gi2204102Redox processes234.1 (15.4)6.40 (5.17)15.1
GST (At)At1g78340Redox processes234.1 (25.2)6.40 (4.99)5.5
30Glyceraldehyde-3-P dehydrogenase (At)At1g13440Carbohydrate metabolism1041.5 (36.9)6.56 (6.79)27.2