Table IV.

Metabolites identified by GC-MS in bean roots from −P- and +P-treated plants

RI, ExpectedRI, sdResponse Ratio −P to +Pa
Amino acids
    Gly1,304.5−0.17−3.3
    β-Ala1,424.7−0.121.4
    Ser1,252.90.095.2
    Asp1,420.50.072.1
    Asn1,665.8−0.0220.0
    4-Aminobutyric acid1,526.5−0.151.9
    Thr1,290.9−0.023.3
    Pro1,298.0−0.161.7
    Glu1,615.4−0.102.1
    Gln1,767.6−0.05−5.0
    Arg (citrulline)b1,814.40.072.8
    Leu1,151.00.523.4
    Lys1,847.3−0.023.5
    Phe1,553.10.155.0
N compounds
    Putrescine (agmatine)b1,737.2−0.241.4
    Allantoin2,067.30.291.6
    Spermidine2,251.1−0.221.6
    Urea1,235.60.06−5.0
Organic acids
    Oxalic acid1,116.80.90−2.5
    Malonic acid1,195.00.30−2.5
    Succinic acid1,310.20.021.4
    Malic acid1,477.30.11−1.1
    2-Methylmalic acid1,464.3−0.091.3
    Shikimic acid1,792.50.051.6
Polyhydroxy acids
    Erythronic acid-1,4-lactone1,426.90.08−1.3
    Galactonic acid-1,4-lactone1,877.4−0.312.5
Polyols
    Threitol1,485.1−0.122.1
    Arabitol1,707.8−0.193.4
    Myoinositol2,083.9−0.273.1
Sugars
    Xyl1,651.6−0.121.9
    Gal1,874.70.162.2
    Fru1,856.2−0.242.8
    Fru1,865.9−0.233.2
    Man1,869.0−0.173.5
    Suc2,629.6−0.281.2
MSTscd
    [516; 1H-Indole-2,3-dione, 1-(tert-butyldimethylsilyl)-5-isopropyl-, 3-(O-methyloxime)]1,691.2−0.04−2.0
    [771; α-d-Methylfructofuranoside (4TMS)]1,760.9−0.021.6
    [802; Methylcitric acid (4TMS)]1,909.57−0.062.2
    [926; Galactosylglycerol (6TMS)]2,297.2−0.125.9
    [802; Gulose (5TMS)]2,424.30.262.0
    [964; Trehalose (8TMS)]2,678.40.027.3
    [882; Melibiose (8TMS)]3,092.9−0.273.5
  • a The response ratio of average −P root response compared with average +P root response is listed (t test significance of P ≤ 0.05 is indicated by bold format of the response ratio). For ratios lower than 1, the inverse of the ratio was estimated and the sign was changed.

  • b Represents the sum of two or more metabolites.

  • c Reference substance not yet available.

  • d MSTs are characterized by match factor and mass spectral hit.