Table V.

Primers and conditions used for semiquantitative RT-PCR

Target GeneEST/TC No.GenBank Accession No. of ESTForward Primer (5′–3′)Reverse Primer (5′–3′)Product SizeAnnealing Tm/Cycles
bp
PeroxidaseTC397CV542921CCA ACC AAA CAC TTG CCA ATGGAG TAG TAG GCC TTG TCG AAT31358°C/20
Glycolipid transfer protein-likeTC1903CV543709GTT GTT CTC AGT CTG CGA TCATAT TGG AGT GGA TGG CAA CGA75160°C/25
Translationally controlled tumor proteinTC63CV542788CGC TCC GCA CCA GTT ATC AGGA TCA GTG GCA CCG TCC TTG52860°C/25
No BLAST hitRTS_103_E03CV541966GGC TTC AAA ATC CTC ACG CGCT TGT TGC TAT CTC AAA CGC21460°C/20
UDP-Glc-6-dehydrogenaseTC1804CV541174GGC TTT ATG TTC TTC TAT GTTTGA AAC CCT CAA ATA TTA CTC13852°C/25
Cytosolic aldehyde dehydrogenaseTC733CV542619CCG TGT GCT CGT TCA AGATC TCC TCC TGC ACT ATT CTC28252°C/25
Senescence-related dihydroorotate dehydrogenaseRTS_109_B06EH792671ATA GCA CTT GGG AAG GTG ATGGTCTTTGTCAGATTG CAAGCC35955°C/25
ACC oxidaseTC280CV542243TGG CAC CAA AGT TAG CAA CTAATT CTG GTG CCA TCC GTT TGA27057°C/25
Hypothetical proteinTC1992CV542243AAC AGT CAA AAG TAT CAC ACGGAT TCA GGG TTT CAG TAT AGG21955°C/25
Isocitrate dehydrogenase (NADP)TC1864CV541923CCC TGA CGG AAA GAC TAT TGAACC TTT ACT TTC TGC GGT GCC59360°C/20
S-adenosyl Met decarboxylaseTC83CV543847TAC TTG GGA CTG TTG TTA TCATAA ACT CAC GGG TAT AGC TTG1,58960°C/20
Ribosomal protein 40 STC1613CV543229TTT CTA GGG TTT CGC TCG GTATAA ACA GAA ACA AAC GCC ATA60060°C/25
Transporter-like proteinTC2875CV543019TGC TTC TTC GCC ACA ACT CAACCT TCC AAG ATG TAG CCG TCA47660°C/25
Trehalose-6-P synthaseRTS_114_F03CV542030TTT CTA AGA ATG AAG CGA GATGAT CCA TCA GTT GTT TCG GTA11652°C/25
Phosphoribosylanthranilate transferaseRTS_139_D07CV543777TGG CCA TGC AGA TTG TGGGA TTA ACA TTA TGC GAT GAA14452°C/25
Caffeine-induced death protein 1RTS_143_D10CV544078AAG CAA AAG CAA TTA GTG GCATAGCAAGGCAAACAT CAATG13457°C/25
AMP diaminaseTC1176CV543772GAC ACT TAT GCC GGT TAT TGGC GAT CAC ACC ATG CGT TAC13652°C/25
Multidrug resistance protein 11TC2799EH791098CAG CAG TGT GAA ATT AAT GGCGTT TTT GGG GGT GGA TTG CAT11157°C/25
UbiquitinTC29CV543388CCA TAA CTC TTG AGG TGG AGA GCTT CCC AGT CAA GGT CTT GAC48055°C/25