Table II.

Analysis of confirmed mutant alleles

Mutant AlleleConfirmed Insertion SiteaSiliques ScreenedSeeds ScreenedAborted SeedsbTop HalfcAverage Seed SizeTerminal Phenotype
%μm
hisn2-1Exon 5 (5)2589816.0***71.5***320Preglobular
501,99720.8***62.9***Preglobular
hisn2-2Exon 5 (5)501,67216.0***69.3***220Preglobular
602,34619.9***65.2***Preglobular
hisn2-3Promoter502,11723.955.9**340Preglobulard
hisn3-1Exon 1 (7)502,16821.7***72.9***270Preglobular
602,20122.0**66.8***Preglobular
hisn3-2Exon 5 (7)502,09220.7***72.3***280Preglobular
hisn4-1Exon 1 (19)502,05921.8**64.7***380Preglobular
451,83020.0***65.0***Preglobular
hisn4-2Intron 14 (18)502,11423.465.8***330Preglobular
501,92620.9***64.5***Preglobular
hisn4-3Intron 17 (18)502,16123.558.7***330Preglobular
hisn6a-1Exon 2 (8)501,78728.7***53.2440Preglobulare
251,05325.054.4340Preglobular
hisn6a-2Exon 7 (8)251,22825.459.2***370Preglobular
1039623.455.6Preglobular
hisn8Exon 10 (11)1104,756[24.5]fN.D.<100Aborted ovule
  • a Based on alignment of flanking sequence; total number of exons or introns noted in parentheses.

  • b Present in heterozygous siliques; 25% expected; significant deviation: **, P < 0.01; ***, P < 0.001.

  • c Aborted (homozygous mutant) seeds in top half of heterozygous siliques; 50% expected. Significant deviation indicates probable effect of mutation on pollen tube growth. N.D., Not determined.

  • d Occasional seeds with cotyledon defects of unknown origin also found in this line and included in totals.

  • e Occasional seeds with delayed phenotype attributed to heterozygotes included in totals for this population.

  • f Percentage of aborted ovules and early aborted seeds combined.