Table IV.

Distribution of heterozygous seeds in siliques produced after crossing hisn3-1 heterozygotes (♂) to wild type (♀)

Silique AnalyzedTotal SeedsResulting Plants ScreenedHeterozygous PlantsaTop Halfb
%
1464245.273.7**
2444452.352.2
3464353.556.5
4454240.776.5**
5453444.173.3**
6383732.4*75.0**
7493542.866.7*
8424131.7*76.9***
9342948.371.4*
10433348.556.2
11433847.444.4
12393250.062.5
13453745.964.7
Total55948744.8*64.2***
  • a Expected 50% if male transmission of the mutant allele was not reduced during the cross. Significant deviation: *, P < 0.05.

  • b Heterozygous seeds present in top half of each silique as determined by phenotyping the resulting plants; 50% expected if mutation has no effect on male transmission. A significant deviation (*, P < 0.05; **, P < 0.01; ***, P < 0.001) indicates a negative effect of the hisn3-1 mutation on pollen tube growth.