Table VIII.

Senescence of mutant seedlings transplanted from His mediaa

Mutant AlleleNo. TestedSenescent
%
Seedlings transplanted to soil
    hisn2-13016.7
    hisn3-14831.3
    hisn4-14219.1
    hisn6a-13920.5b
Seedlings transplanted to basal medium
    hisn4-14126.8
    hisn6a-1310.0c
    hisn87714.3
  • a Mature seeds from rescued heterozygotes were germinated on a medium with 1 mm His. The resulting seedlings were transplanted after 2 to 3 weeks in culture and the number of senescent plants determined after 2 to 3 weeks without His.

  • b Mutant plants were smaller than normal but remained viable much longer than with other rescued knockouts.

  • c Mutant plants could not be identified by phenotype until several weeks later.