Table I.

Cuticular wax composition of wild-type and mutant inflorescence stems

Total wax amounts and coverage of individual compound classes (μg/cm2) are given as mean values ± se for individual compound classes (n = 9 in three batches). Different letters within the compound class show that wax loads differed significantly between respective lines (mixed-effect ANOVA; Tukey-Kramer posthoc test, P < 0.05). Trace and 0.0 signify values that were below 0.05 μg/cm2. Mixed-effect ANOVA (α = 0.05) was conducted using treatment as a fixed effect and batch nested within treatment as a random effect. Pertinent results for each compound class were as follows: Total load, F3, 8.132 = 11.083, P = 0.003, β = 0.026; alkanes, F3, 8.108 = 4.399, P = 0.041, β = 0.337; secondary alcohols, F3, 8.315 = 13.351, P = 0.002, β = 0.009; ketones, F3, 8.348 = 51.738, P < 0.0005, β < 0.0005; aldehydes, F3, 8.297 = 0.905, P = 0.479, β = 0.827; fatty acids, F3, 8.520 = 3.970, P = 0.049, β = 0.374; primary alcohols, F3, 8.141 = 1.899, P = 0.207, β = 0.674; esters, F3, 8.351 = 4.900, P = 0.030, β = 0.280; triterpenoids, F3, 8.185 = 2.475, P = 0.134, β = 0.585; not identified, F3, 8.241 = 1.686, P = 0.244, β = 0.706.

LineTotal LoadAlkanesSecondary AlcoholsKetonesAldehydesFatty AcidsPrimary AlcoholsEstersTriterpenoidsNot Identified
Wild type (Col-0)25.1 ± 0.6 a9.6 ± 0.4 a2.4 ± 0.1 a6.0 ± 0.2 a1.1 ± 0.2 a0.4 ± 0.1 a2.4 ± 0.3 a1.0 ± 0.1 a0.6 ± 0.1 a1.8 ± 0.2 a
mah1-115.7 ± 1.0 b11.2 ± 0.7 b0.1 ± 0.0 bTrace b0.4 ± 0.1 b0.1 ± 0.0 b1.3 ± 0.1 b0.9 ± 0.1 a0.5 ± 0.1 a1.2 ± 0.2 b
mah1-221.2 ± 0.6 c11.8 ± 0.3 b1.7 ± 0.1 c1.5 ± 0.1 c1.2 ± 0.2 a0.4 ± 0.1 a1.6 ± 0.3 b0.9 ± 0.1 a0.6 ± 0.1 a1.4 ± 0.2 b
mah1-327.1 ± 1.1 a13.7 ± 0.5 c2.5 ± 0.1 a3.2 ± 0.2 d0.9 ± 0.2 ab0.4 ± 0.1 a2.4 ± 0.2 a1.5 ± 0.2 b0.8 ± 0.0 b1.6 ± 0.2 ab