Table II.

Distribution of extractable radioactivity in P. patens protonema tissue (150–242 mg FW) after feeding with [2-3H]iPR (60 pmol/mL) in a volume of 4 mL

Percentages related to total amount of extractable radioactivity are given in brackets. iPRDP, Isopentenyladenosine diphosphate; iPRTP, isopentenyladenosine triphosphate. d.l., Detection limit (approximately 0.01 pmol).

GenotypeTimeiPRiPiP NucleotidesDegradation Products
iPRMPiPRDP, iPRTP
hpmol/100 mg [%]
Wild type40.1 [3.7]0.1 [4.0]0.5 [18.9]0.5 [20.0]1.4 [53.4]
200.6 [7.5]1.3 [17.8]1.2 [15.6]0.7 [9.3]3.7 [49.8]
tCKX74<d.l.<d.l.<d.l.<d.l.14.9 [100.0]
20<d.l.<d.l.<d.l.0.3 [2.9]11.2 [97.1]
tCKX1640.2 [3.0]0.3 [5.4]0.6 [10.5]0.3 [5.6]4.3 [75.5]
20<d.l.<d.l.0.2 [2.0]0.4 [3.6]10.0 [94.4]