Table I.

Genetic elements in entry clones

ClassElement(s)AbbreviationsbRecipient pDONREntry Cloneatt Sites
PromoterspNOS1pDONR P4-P1RpEN-L4-1-R1attL4-attR1
pCaMV 35S2pDONR P4-P1RpEN-L4-2-R1attL4-attR1
pROLD3pDONR P4-P1RpEN-L4-3-R1attL4-attR1
pCsVMV4pDONR P4-P1RpEN-L4-4-R1attL4-attR1
pUBI-1L, first exon and intronUBILpDONR P4-P1RpEN L4-UBIL-R1attL4-attR1
pUBI-1SUBISpDONR P4-P1RpEN-L4-UBIS-R1attL4-attR1
Reporter enzymesGUS, no stopSpDONR 221pEN-L1-S-L2attL1-attL2
GUS, intronSIpDONR 221pEN-L1-SI-L2attL1-attL2
GUS, no stopSpDONR P2R-P3pEN-R2-S-L3attR2-attL3
GUS, with stopS*pDONR P2R-P3pEN-R2-S*-L3attR2-attL3
LUC+L+pDONR 221pEN-L1-L+-L2attL1-attL2
Ω leader, LUC+OL+pDONR 221pEN-L1-OL+-L2attL1-attL2
LUC+L+pDONR P2R-P3pEN-R2-L+-L3attR2-attL3
Fluorescent tagsGFPFpDONR 221pEN-L1-F-L2attL1-attL2
GFP, intronFIpDONR 221pEN-L1-FI-L2attL1-attL2
NLS, GFP, intronNFIpDONR 221pEN-L1-NFI-L2attL1-attL2
ER, GFPERFpDONR P4-P3pEN-L4-ERF-L3attL4-attL3
YFPYpDONR 221pEN-L1-Y-L2attL1-attL2
CFPCpDONR 221pEN-L1-C-L2attL1-attL2
DsRedRpDONR 221pEN-L1-R-L2attL1-attL2
NLS-GFPNFpDONR 221pEN-L1-NF-L2attL1-attL2
NLS-YFPNYpDONR 221pEN-L1-NY-L2attL1-attL2
NLS-CFPNCpDONR 221pEN-L1-NC-L2attL1-attL2
NLS-DsRedNRpDONR 221pEN-L1-NR-L2attL1-attL2
GFPFpDONR P2R-P3pEN-R2-F-L3attR2-attL3
YFPYpDONR P2R-P3pEN-R2-Y-L3attR2-attL3
CFPCpDONR P2R-P3pEN-R2-C-L3attR2-attL3
DsRedRpDONR P2R-P3pEN-R2-R-L3attR2-attL3
Mini 35S, NLS, GFP, intronaM2NFIpDONR 221pEN-L1-M2NFI-L2attL1-attL2
TerminatorstNOS6pDONR P2R-P3pEN-R2-6-L3attR2-attL3
tCaMV 35S7pDONR P2R-P3pEN-R2-7-L3attR2-attL3
tOCS8pDONR P2R-P3pEN-R2-8-L3attR2-attL3
tG79pDONR P2R-P3pEN-R2-9-L3attR2-attL3
Two-component elementsLHOATG4pDONR 221pEN-L1-LHOATG4-L2attL1-attL2
LHGROATG4pDONR 221pEN-L1-LHGROATG4-L2attL1-attL2
pOp6M2pDONR P4-P1RpEN-L4-pOp6M2-R1attL4-attR1
  • a This entry clone carries a hybrid sequence with a minimal CaMV 35S promoter upstream of an ORF coding for a NLS fused to GFP and interrupted by an intron.

  • b Abbreviation used in plasmid names.