Table II.

List of cutin monomers identified after GC-MS analysis with their respective concentrations in dso-3 mutants and wild-type plants

Data presented here are the average of three replicates.

nCompound ClassWild Typesddso-3sdP ValueChange versus Wild Type
mean ng/cm2mean ng/cm2
Alkan-1-oic acids
    1C18 octadecenoic acid(1)1.480.100.760.11P < 0.01
    2C18 octadeca(dien+trien)oic acid(2,3)17.371.546.781.01P < 0.01
    3C22 docosanoic acid3.530.431.250.07P < 0.01
    4C24 tetracosanoic acid4.200.361.940.03P < 0.01
Total26.582.4310.731.21
2-Hydroxy acids
    5C16 2-hydroxy-hexadecanoic acid4.570.061.750.08P < 0.01
    6C20 2-hydroxy-eicosanoic acid1.510.120.530.06P < 0.01
    7C22 2-hydroxy-docosanoic acid7.920.083.490.15P < 0.01
    8C23 2-hydroxy-tricosanoic acid1.200.110.560.06P < 0.01
    9C24 2-hydroxy-tetracosenoic acid(1)23.770.565.980.38P < 0.01
    10C24 2-hydroxy-tetracosanoic acid28.790.6410.170.09P < 0.01
    11C25 2-hydroxy-pentacosenoic acid(1)0.940.120.270.01P < 0.01
    12C25 2-hydroxy-pentacosanoic acid1.400.080.660.03P < 0.01
    13C26 2-hydroxy-hexacosenoic acid(1)2.300.110.740.02P < 0.01
    14C26 2-hydroxy-hexacosanoic acid9.450.044.310.13P < 0.01
Total81.851.9428.461.01
ω-Hydroxy acids
    15C16 16-hydroxy-hexadecanoic acid1.950.090.380.04P < 0.01
    16C17 17-hydroxy-heptadecanoic acid1.900.190.310.01P < 0.01
    17C18 18-hydroxy-octadecadienoic acid(2)1.800.110.600.10P < 0.01
    18C18 18-hydroxy-octadecatrienoic acid(3)1.950.160.420.09P < 0.01
Total7.590.551.710.25
α,ω-Dicarboxylic acids
    19C16 hexadecane-(1,16)-dioic acid13.891.411.910.12P < 0.01
    20C18 octadecane-(1,18)-dioic acid2.980.260.590.02P < 0.01
    21C18 octadecen-(1,18)-dioic acid(1)9.160.891.690.06P < 0.01
    22C18 octadecadien-(1,18)-dioic acid(2)29.941.886.690.73P < 0.01
Total55.964.4310.880.94
Midchain oxygenated fatty acids21.031.355.570.31
Unknown aliphatics10.420.463.670.28
Unidentified compounds8.160.462.940.38
Sum total211.5811.6363.964.38