Table I.

Ascorbate in tomato leaf and fruit of P_35S:Slgalldh^RNAi transgenic and control plants

Reduced (AsA) and total ascorbate (Tot Asc) contents of P_35S:Slgalldh^RNAi line 8, line 5, and control plants were measured in young developing leaves from seedlings at the indicated DAS and in developing fruits at the indicated days postanthesis (DPA). Apoplastic ascorbate content was measured in the fully expanded fourth leaf of 6-week-old plants. The reduced/total ascorbate ratio was determined for all the samples. Data represent mean ± sd of six individual plants with two repeats per plant. The superscript a (^a) indicates values that were determined by the t test to be significantly different (P < 0.05) from control.

Plant Stage (DAS)		0_(Seed)	15	21	28	42
Leaf		μmol g⁻¹ FW
AsA	Control	2.4 ± 0.2	1.5 ± 0.1	2.5 ± 0.3	2.6 ± 0.3	1.8 ± 0.1
	Line 8	2.5 ± 0.3	1.2 ± 0.1^a	1.1 ± 0.1^a	1.3 ± 0.1^a	1.4 ± 0.2^a
	Line 5	2.5 ± 0.2	1.1 ± 0.2^a	1.2 ± 0.2^a	1.4 ± 0.1^a	1.3 ± 0.2^a
Tot Asc	Control	2.5 ± 0.3	2.9 ± 0.2	4.5 ± 0.5	8.7 ± 0.7	8.5 ± 0.3
	Line 8	2.6 ± 0.2	2.9 ± 0.3	4.9 ± 0.5	6.1 ± 0.4	8.1 ± 0.3
	Line 5	2.6 ± 0.2	2.8 ± 0.3	5.5 ± 0.5	7.7 ± 0.1	8.1 ± 0.4
Ratio	Control	0.9	0.5	0.5	0.3	0.2
	Line 8	1	0.4	0.2	0.2	0.2
	Line 5	1	0.4	0.2	0.2	0.2
Fruit Stage (DPA)		10	20	30	38	42
Fruit
AsA	Control	0.3 ± 0.1	0.4 ± 0.1	1.3 ± 0.1	1.7 ± 0.1	1.7 ± 0.2
	Line 8	0.5 ± 0.1^a	0.9 ± 0.1^a	1.6 ± 0.2^a	1.6 ± 0.7	2.0 ± 0.2
	Line 5	0.6 ± 0.1^a	0.7 ± 0.1^a	1.6 ± 0.2^a	1.8 ± 0.2	1.9 ± 0.2
Tot Asc	Control	1.6 ± 0.1	1.6 ± 0.1	1.6 ± 0.2	1.7 ± 0.1	2.0 ± 0.1
	Line 8	2.0 ± 0.1^a	1.8 ± 0.3	1.7 ± 0.2	1.6 ± 0.7	2.2 ± 0.2
	Line 5	1.8 ± 0.1^a	1.6 ± 0.1	1.6 ± 0.2	2.0 ± 0.2	2.1 ± 0.2
Ratio	Control	0.2	0.2	0.8	1	0.9
	Line 8	0.2	0.5	0.9	0.9	0.9
	Line 5	0.3	0.4	0.9	0.9	0.9

Leaf Apoplast	Control	Line 8	Line 5
	nmol g⁻¹ FW
AsA	85 ± 60	111 ± 10	121 ± 47
Tot Asc	648 ± 49	402 ± 47^a	370 ± 104^a
Ratio	0.1	0.3	0.3