Table I.

Constructs generated for SE plasma membrane mCitrine labeling

FamilyNameAKATAIR LocusaType of ClonebPredicted
Fusion LengthcFusion MasscTMDsd
amino acidskD
RCI2RCI2AAt3g05880cDNA/genomic307342
RCI2BAt3g05890genomic309342
PIPPIP1;1PIP1AAt3g61430cDNA/genomic541596
PIP1;2PIP1BAt2g45960cDNA/genomic540596
PIP1;3PIP1CAt1g01620cDNA541596
PIP2;1PIP2AAt3g53420cDNA/genomic542596
PIP2;2PIP2BAt2g37170cDNA/genomic540596
PIP2;7PIP3At4g35100cDNA/genomic535586
SUCSUC2At1g22710cDNA/genomic7688312
  • a Rhee et al. (2003).

  • b Clones of each gene were generated from either genomic or cDNA templates, or both, beginning with the start codon and including the full 3′-UTR.

  • c Predicted fusion length and mass include Ala-10 linker (Supplemental Fig. S1). Soluble mCitrine (239 amino acids) has a predicted mass of 27 kD.

  • d The evidence for two transmembrane domains in the RCI2 protein family is still circumstantial (Nylander et al., 2001; Kroemer et al., 2004).