Table I.

Validation of microarray data by quantitative RT-PCR for 11 up-regulated and four down-regulated genes

GenBank AccessionGene ProductArray Ratioa (Cmm/Mock)qRT-PCR Ratiob (Cmm/Mock)
U89256Pti5, ERF/AP2 transcription factor163193
AF272366Verticillium wilt disease R protein883
AI776170Cys protease81116
AJ133600Extensin20105
BG629612Chitinase55317
AY359965EIX receptor 1109
AW219676Wound-induced protein Sn-1821
AY093595Osmotin-like protein102
AW032581KNOTTED1 transcription factor924
BG629373Zeatin glucosyl transferase66
CN385925Patatin-like protein 354
AW622607Nitrate reductase0.50.6
AW647641Peroxidase0.50.9
BG630282Probable protein kinase0.40.4
BF176599Pro-rich protein0.20.2
  • a Ratio between expression in Cmm- and mock-inoculated stems as estimated by microarray analysis at 8 dpi. Data represent the mean of two biological replicates

  • b Ratio between expression in Cmm- and mock-inoculated stems as estimated by qRT-PCR at 8 dpi. Data represent the mean of three biological replicates.