Table I.

In vivo protein-protein interaction test results

The indicated GAL4-BD and GAL4-AD fusion plasmids were cotransformed into yeast strain PJ69-4A. +, Growth on minimal medium lacking Ade and blue color in the β-galactosidase assay. At least four independent transformants were tested for each pair. n.d., Data for the indicated plasmid pair were not determined; None, negative control plasmids lacking any maize sequence. Positive controls p53 and SV40 large T antigen (LTA) are described in “Materials and Methods.”

BD FusionAD Fusion
BEIIaBEIIbBEISSISSIII1–181SSIII1–367SSIII366–648SSIIIHDNoneLTA
BEIIa+++++++
BEIIbn.d.n.d.
BEIn.d.n.d.
SSI++n.d.
SSIII1–181n.d.n.d.
SSIII1–367++++n.d.
SSIII366–648n.d.n.d.n.d.
SSIIIHDn.d.+n.d.n.d.n.d.
Nonen.d.
p53n.d.n.d.n.d.n.d.n.d.n.d.n.d.+