Table III.

Summary of observed physical interactions

Results are noted for coimmunoprecipitation (Co-IP), affinity purification (Affinity), and in vivo protein-protein interaction tests (Y2H). For each method, the results of the test performed in both possible orientations are noted (A, B). nd, Either the test was not performed or the results were not unequivocal.

Interaction PairMethod
Co-IPaAffinitybY2Hc
ABABAB
SSI/SSIIa+++ndndnd
SSI/SSIIInd++nd+
SSI/BEIIa+++++
SSI/BEIIb+nd+nd
SSI/BEI+ndnd
SSIIa/BEIIa+ndnd+ndnd
SSIIa/BEIIb+ndndndndnd
SSIII/BEIIandndndnd++
  • a “A” indicates that antibodies specific for the first member of the binding pair were used as the precipitating agent, and “B” indicates that antibodies specific for the second member were used for precipitation.

  • b “A” indicates that the first member of the binding pair was the solid phase ligand, and “B” indicates the second member was in the solid phase.

  • c “A” indicates that the first listed member of the binding pair was fused to GAL4-AD, and “B” indicates the second member was fused to GAL4-AD.