Table I.

Enzyme activities in antisense mitochondrial citrate synthase lines

Activities were determined in 6-week-old fully expanded source leaves harvested 6 h into the photoperiod. Data presented are mean ± se of measurements from six independent plants per genotype; values set in bold type were determined by the t test to be significantly different (P < 0.05) from the wild type.

Wild TypeCS40CS22CS25CS45
Enzymesnmol min−1 g−1 FW
    AGPase1211.73 ± 22.061217.43 ± 96.19902.73 ± 78.71890.54 ± 130.04923.49 ± 122.81
    Fru-1,6-bisphosphatase (cytosolic)140.34 ± 16.7395.05 ± 14.0089.81 ± 17.3555.35 ± 4.4151.91 ± 5.28
    Gln synthetase1868.23 ± 312.56967.12 ± 238.28611.12 ± 227.54356.57 ± 129.11257.81 ± 61.84
    NADP-isocitrate dehydrogenase1032.39 ± 110.07674.01 ± 45.48600.4 ± 99.24690.08 ± 99.32711.17 ± 86.66
    Malate dehydrogenase initial (NADP)51.31 ± 3.2340.64 ± 3.5933.31 ± 5.4047.6 ± 7.2654.03 ± 3.56
    Malate dehydrogenase total (NADP)110.28 ± 8.7989.08 ± 5.7478.23 ± 5.58113.83 ± 5.89113.05 ± 7.48
    Nitrate reductase389.48 ± 33.13206.07 ± 26.42237.57 ± 47.0150.17 ± 35.25155.27 ± 49.55
    PEP carboxylase745.56 ± 38.31618.41 ± 31.83528.13 ± 30.99620.49 ± 20.32621.88 ± 28.12
    Phosphoglucomutase7208.23 ± 314.614990.02 ± 267.944570.91 ± 321.146375.39 ± 342.316082.76 ± 399.16
    Shikimate dehydrogenase582.86 ± 49.34471.48 ± 44.28403.21 ± 32.41415.18 ± 35.33426.8 ± 27.17