Enzyme activities in antisense mitochondrial citrate synthase lines
Activities were determined in 6-week-old fully expanded source leaves harvested 6 h into the photoperiod. Data presented are mean ± se of measurements from six independent plants per genotype; values set in bold type were determined by the t test to be significantly different (P < 0.05) from the wild type.
| Wild Type | CS40 | CS22 | CS25 | CS45 | |
|---|---|---|---|---|---|
| Enzymes | nmol min−1 g−1 FW | ||||
| AGPase | 1211.73 ± 22.06 | 1217.43 ± 96.19 | 902.73 ± 78.71 | 890.54 ± 130.04 | 923.49 ± 122.81 |
| Fru-1,6-bisphosphatase (cytosolic) | 140.34 ± 16.73 | 95.05 ± 14.00 | 89.81 ± 17.35 | 55.35 ± 4.41 | 51.91 ± 5.28 |
| Gln synthetase | 1868.23 ± 312.56 | 967.12 ± 238.28 | 611.12 ± 227.54 | 356.57 ± 129.11 | 257.81 ± 61.84 |
| NADP-isocitrate dehydrogenase | 1032.39 ± 110.07 | 674.01 ± 45.48 | 600.4 ± 99.24 | 690.08 ± 99.32 | 711.17 ± 86.66 |
| Malate dehydrogenase initial (NADP) | 51.31 ± 3.23 | 40.64 ± 3.59 | 33.31 ± 5.40 | 47.6 ± 7.26 | 54.03 ± 3.56 |
| Malate dehydrogenase total (NADP) | 110.28 ± 8.79 | 89.08 ± 5.74 | 78.23 ± 5.58 | 113.83 ± 5.89 | 113.05 ± 7.48 |
| Nitrate reductase | 389.48 ± 33.13 | 206.07 ± 26.42 | 237.57 ± 47.01 | 50.17 ± 35.25 | 155.27 ± 49.55 |
| PEP carboxylase | 745.56 ± 38.31 | 618.41 ± 31.83 | 528.13 ± 30.99 | 620.49 ± 20.32 | 621.88 ± 28.12 |
| Phosphoglucomutase | 7208.23 ± 314.61 | 4990.02 ± 267.94 | 4570.91 ± 321.14 | 6375.39 ± 342.31 | 6082.76 ± 399.16 |
| Shikimate dehydrogenase | 582.86 ± 49.34 | 471.48 ± 44.28 | 403.21 ± 32.41 | 415.18 ± 35.33 | 426.8 ± 27.17 |