Table II.

Fatty acid contents in wild-type and LEC1-OXi seedlings treated with different concentrations of estradiol

Ten-day-old wild-type and pER8-LEC1 seedlings germinated and grown on MS medium containing different concentrations of estradiol as indicated were used for fatty acid analysis as described in “Materials and Methods.” Treatment with 25 μm estradiol did not significantly alter the fatty acid level compared with treatment with 10 μm estradiol (data not shown). Data reported are mean values of three independent experiments (three biological repeats) ± se. All values are expressed as μg mg−1 fresh weight. ND, Not detectable.

Fatty AcidEstradiol Level
0 μm1 μm5 μm10 μm
Col-0LEC1-OXiCol-0LEC1-OXiCol-0LEC1-OXiCol-0LEC1-OXi
16:00.50 ± 0.040.55 ± 0.020.60 ± 0.000.72 ± 0.050.57 ± 0.080.77 ± 0.020.52 ± 0.020.70 ± 0.15
18:00.10 ± 0.030.12 ± 0.000.12 ± 0.030.19 ± 0.020.13 ± 0.060.24 ± 0.050.11 ± 0.030.21 ± 0.01
18:10.19 ± 0.010.16 ± 0.010.10 ± 0.040.45 ± 0.190.12 ± 0.010.64 ± 0.070.09 ± 0.061.17 ± 0.24
18:20.53 ± 0.060.61 ± 0.200.53 ± 0.011.53 ± 0.140.55 ± 0.001.71 ± 0.111.45 ± 0.103.16 ± 0.59
18:31.29 ± 0.131.56 ± 0.111.35 ± 0.122.04 ± 0.141.26 ± 0.172.15 ± 0.221.34 ± 0.093.48 ± 0.38
20:0NDNDND0.09 ± 0.07ND0.11 ± 0.04ND0.12 ± 0.05
20:1NDNDND1.14 ± 0.21ND1.40 ± 0.15ND1.51 ± 0.17
20:2NDNDND0.13 ± 0.06ND0.17 ± 0.05ND0.22 ± 0.04
22:0NDNDND0.01 ± 0.01ND0.03 ± 0.00ND0.03 ± 0.01
22:1NDNDND0.12 ± 0.02ND0.16 ± 0.07ND0.22 ± 0.09
Sum2.61 ± 0.273.00 ± 0.342.70 ± 0.206.42 ± 0.912.63 ± 0.327.38 ± 0.783.51 ± 0.3010.82 ± 1.73