Table III.

Fatty acid compositions in individual seeds and seedlings of wild-type and LEC1-OXi plants

The same batches of wild-type (Col-0) and pER8-LEC1 seeds were used in the analysis. Ten-day-old seedlings germinated and grown in the presence (LEC1-OXi) or absence (Col-0) of estradiol were collected for fatty acid analysis as described in “Materials and Methods.” Randomly collected samples (100 seeds or seedlings per sample) were mixed and then used for extraction of total fatty acids and lipids. Mean values of fatty acid methyl esters in individual seeds or seedlings were calculated by dividing raw data by 100. Data presented are mean values of three independent experiments (three biological repeats) ± se. All values are expressed as μg seed−1 or μg seedling−1 as applicable. ND, Not detectable.

Fatty AcidSeedSeedling
Col-0pER8-LEC1Col-0LEC1-OXi
C16:00.36 ± 0.070.39 ± 0.110.39 ± 0.031.16 ± 0.03
C16:10.02 ± 0.010.02 ± 0.000.02 ± 0.010.01 ± 0.01
C16:30.01 ± 0.000.01 ± 0.010.37 ± 0.080.05 ± 0.08
C18:00.12 ± 0.010.11 ± 0.010.07 ± 0.010.30 ± 0.03
C18:10.48 ± 0.060.51 ± 0.140.09 ± 0.101.38 ± 0.11
C18:21.31 ± 0.121.28 ± 0.210.45 ± 0.042.55 ± 0.12
C18:31.06 ± 0.231.15 ± 0.161.16 ± 0.123.78 ± 0.43
C20:00.05 ± 0.010.04 ± 0.01ND0.18 ± 0.05
C20:10.93 ± 0.110.91 ± 0.070.01 ± 0.002.09 ± 0.22
C20:20.07 ± 0.020.05 ± 0.01ND0.23 ± 0.04
C22:10.06 ± 0.020.04 ± 0.02ND0.28 ± 0.07
Sum4.47 ± 0.664.51 ± 0.802.56 ± 0.3912.01 ± 1.19