Table I.

Genes associated with amino acid metabolism and secretory processes that are preferentially expressed in N. benthamiana or S. pennellii leaf trichomes

The genes that are represented have a putative function in metabolism or secretion, according to their annotation. Expression ratios refer to the level of expression of any given gene in trichomes compared with its expression in underlying leaf tissues. Annotations correspond to the description of the protein that is most closely related to the predicted product of each gene. Genes associated with BCAA biosynthesis are shown in boldface.

Unigene NumberExpression RatioAnnotation
N. benthamiana
Amino acid metabolism
    SGN-U23272210.12-Isopropylmalate synthase [S. pennellii]
    SGN-U2125948.0His decarboxylase [Solanum esculentum]
    SGN-U2125957.4His decarboxylase [S. esculentum]
    SGN-U2218647.0Putative dihydrolipolyacyltransferase subunit of the BCKD complex [Oryza sativa]
    SGN-U2178006.2Putative 3-isopropylmalate dehydratase large subunit [O. sativa]
    SGN-U2160215.13-Isopropylmalate dehydrogenase [Brassica napus]
    SGN-U2186572.7Pyridoxamine-phosphate oxidase [Arabidopsis]
    SGN-U2166202.6Asp aminotransferase, cytoplasmic (transaminase A)
    SGN-U2159002.4Putative Thr dehydratase/deaminase [O. sativa]
    SGN-U2210782.1Orn decarboxylase [S. esculentum]
    SGN-U2210332.1Isovaleryl-CoA dehydrogenase [S. tuberosum]
Putative secretion
    SGN-U2315406.6Pleiotropic drug resistance-like protein [N. tabacum]
    SGN-U2222186.6Nonspecific lipid transfer protein [S. esculentum]
    SGN-U2320892.8TSW12 (putative lipid transfer protein) [S. esculentum]
    SGN-U2169232.7Putative lipid transfer protein, glycosylphosphatidylinositol-anchored [Cicer arietinum]
    SGN-U2127362.6Nonspecific lipid transfer protein [S. esculentum]
    SGN-U2320892.2LETSW12 S. esculentum TSW12 mRNA (putative lipid transfer protein)
S. pennellii
Amino acid metabolism
    SGN-U2218646.5Putative dihydrolipolyacyltransferase subunit of the BCKD complex [O. sativa]
    SGN-U2269084.9Putative dihydrodipicolinate reductase-like protein [O. sativa]
    SGN-U2137683.9Acetolactate synthase SuRB
    SGN-U2159003.5Putative Thr dehydratase/deaminase [O. sativa]
    SGN-U2131633.3CIG1 (Pro oxidase) [N. tabacum]
    SGN-U2131623.3Pro oxidase/dehydrogenase 2 [N. tabacum]
    SGN-U2146083.0DIN4 (DARK-INDUCIBLE4); 3-methyl-2-oxobutanoate dehydrogenase [Arabidopsis]
    SGN-U2150852.9Gln synthetase (Glu-ammonia ligase)
Putative secretion
    SGN-U2222182.7Nonspecific lipid transfer protein [S. esculentum]
    SGN-U2127362.5Nonspecific lipid transfer protein [S. esculentum]