Table II.

Trichome/leaf gene expression ratios determined by microarray analysis and real-time RT-PCR

Gene ProductAliasN. benthamianaS. pennellii
MicroarrayReal-Time RT-PCRaMicroarrayReal-Time RT-PCR
RatioPSet 1Set 2RatioPSet 1Set 2
RatiosdRatiosdRatiosdRatiosd
BCAA synthesis
    Acetolactate synthaseSuRAbNCc3.9d0.099.02.028.45.1
    Thr dehydratase/deaminaseTD2.40.053.50.093.91.016.93.2
    2-Isopropylmalate synthaseIPMS ANC0.20.040.40.11.90.091.40.42.80.8
IPMS B10.10.020.40.040.60.1NC1.70.41.00.2
IPMS C14.83.427.42.0
    3-Isopropylmalate dehydrogenaseIPMD5.10.043.40.35.50.41.70.104.20.911.52.1
    3-Isopropylmalate dehydratase (large subunit)IPDS-L6.20.030.40.10.60.01.90.092.40.65.91.2
BCAA degradation
Branched-chain α-keto acid dehydration
    E1-β subunitβ-BCKDNC14.13.04.60.53.0d0.094.10.99.81.9
    E2 subunitBCKD E27.00.034.60.713.01.36.5d0.0911.83.248.215.3
Fatty acid elongation
    β-Ketoacyl-ACP synthase IIIAKAS IIIANC1.50.092.80.64.20.8
    β-Ketoacyl-ACP synthase IKAS IA2.30.72.30.4
KAS IB5.41.319.13.9
KAS IC9.74.22.81.8
Control
    Glyceraldehyde 3-phosphate dehydrogenaseGAPDH0.90.20.60.11.40.41.10.3
  • a Real-time RT-PCR data sets 1 and 2 were obtained from plants grown on two separate occasions, using actin normalization.

  • b TOM2 probe annotated as SuRB (Table I) matches current SGN entry annotated as SuRA (Supplemental Table S7).

  • c NC, No significant change observed.

  • d Data verified with more than one real-time RT-PCR primer pair (Supplemental Table S6).