Metabolism of [1-14C]Gly to 14CO2 and [14C]Ser in darkness by maize leaf discs in a segregating mutant population with varying GO activities
The [14C]Gly and [14C]Ser were separated by HPLC as described in “Materials and Methods.” The mean values are given for each plant material ±se (n = 3).
Plant Material | 14CO2 Released | [14C]Ser Formed | 14CO2 to [14C]Ser |
---|---|---|---|
dpm | mean ratioa | ||
Low GO | 23,900 ± 2,410 | 21,300 ± 1,260 | 1.12 |
Intermediate GO | 22,900 ± 1,730 | 22,400 ± 2,080 | 1.02 |
Wild-type GO | 25,000 ± 5,340 | 29,200 ± 7,510 | 0.86 |
↵a One-way ANOVA indicated no significant effect (P = 0.43) of GO phenotype on the 14CO2 to [14C]Ser ratio. The mean ratio calculated across all phenotypes was 1.02 (se ± 0.07; n = 9).