Quantitative analysis of vacuolar transfers of RCBs, chloroplasts, and stroma-targeted DsRed
RCB and chloroplast columns indicate cell numbers having RCBs or chloroplasts in the vacuole per 100 cell sections observed by LSCM. Intensity of DsRed in the vacuole of wild-type background plants represents the relative intensity of DsRed accumulated in the vacuole as the IDL 5-d + concanamycin A (conA) 20-h criterion. Fluorescence intensity is measured at cell center spots of images captured using the same laser power and detector gain. Values are means ± sd (n = 50). Different letters represent significant difference statistically analyzed by Tukey-Kramer's HSD test (P ≤ 0.01). The correlation coefficient between treatment time and RCB observability is 0.9867.
| Treatment | Span for Treatment | RCB | Whole Cp | Intensity of DsRed in Vacuole | ||||
|---|---|---|---|---|---|---|---|---|
| Wild Type | atg4a4b-1 | Wild Type | atg4a4b-1 | |||||
| Control | 5 d | 0 | 0 | 0 | 0 | 4.1 ± 2.6d | ||
| IDLs | 1 d | 21 | 0 | 0 | 0 | 20.0 ± 8.1c | ||
| 3 d | 49 | 0 | 4 | 0 | 32.6 ± 13c | |||
| 5 d | 66 | 0 | 5 | 0 | 68.3 ± 20b | |||
| 5 d + conA 20 h | 97 | 0 | 43 | 0 | 100.0 ± 37a | |||