Table I.

Putative in vivo sumoylation substrates of Arabidopsis

Criteria for inclusion of a protein were robust detection in preparations, absence from control preparations, and apparent molecular mass, as detailed in the text. n.t., Not tested.

IdentifierCharacteristicsΨKxD/EaIn Vivo Conjugate to:In Vitro Sumoylationb
RNA-dependent processes
    At1g29400AML5; has one RRM2 domain, two RRM1 domains1 (1)SUM3Yes
    At3g56860Contains two RRM1 domains1 (0)SUM1Yes
    At2g43970Contains an LA domain0 (1)SUM1No
    At2g47020Translation release factor1 (2)SUM3n.t.
    At3g60240eIF4G4 (2)SUM3n.t.
Chromatin-related processes
    At1g55300TAF7 (TFIID subunit)0 (0)SUM3, -5Yes
    At2g19480Nucleosome assembly factor2 (0)SUM1Yes
    At5g08450Contains an Rxt3 domain3 (10)SUM3Yes
    At5g39660zfDOF transcription factor1 (1)SUM1n.t.
    At5g43130TFIID subunit2 (4)SUM1n.t.
    At1g27430Contains a GYF domain1 (4)SUM1, -3n.t.
    At1g64330Contains a KIP domain, coiled-coil region2 (1)SUM1, -3n.t.
    At5g13480FY; WD40 repeat protein0 (0)SUM1, -3n.t.
    At5g52300RD29B desiccation induced3 (2)SUM1n.t.
  • a In addition to canonical SUMO attachment sites (Ψ is an aliphatic hydrophobic residue, x indicates any residue, D/E is either Asp or Glu), many proteins contain nearly canonical sequences as further potential SUMO attachment sites (number of such sites is given in parentheses;

  • b The in vitro sumoylation reactions referred to here used SUMO1 and its variant SUMO1 Q90A with identical results.