Table I.

ROS production in embryonic axes during imbibition

H2O2 and superoxide (O2·−) production in embryonic axes isolated from dormant (d) and after-ripened, nondormant (nd) sunflower embryos before imbibition (dry) and from embryos that were imbibed on water for 24 h at 10°C (H2O), or treated for 3 h with 0.1 mm MV (MV) or with gaseous cyanide (HCN) and then transferred for 21 h on water at 10°C. Values are means of five replicates ± sd. DW, Dry weight.

TreatmentDuration of ImbibitionH2O2O2.−
dnddnd
hnmol g DW−1nmol g DW−1
No0 (dry)1.51 ± 0.212.10 ± 0.040.62 ± 0.030.91 ± 0.06
H2O32.12 ± 0.132.96 ± 0.090.63 ± 0.010.98 ± 0.05
HCN32.81 ± 0.124.22 ± 0.20.70 ± 0.061.01 ± 0.06
MV32.73 ± 0.113.11 ± 0.150.70 ± 0.040.99 ± 0.06
H2O242.50 ± 0.193.26 ± 0.170.61 ± 0.051.10 ± 0.06
HCN244.25 ± 0.355.05 ± 0.110.67 ± 0.051.28 ± 0.06
MV244.51 ± 0.175.44 ± 0.220.69 ± 0.031.20 ± 0.02