Table I.

Quantitation of jasmonates and auxins in Arabidopsis tissues

nd, Not detected. Values are the means of three or four independent tissue extractions with se in parentheses.

TreatmentaJA-TrpIAA-TrpIAAJAJA-Ile
pmol/g FW
Root
    Control1.7 (0.3)1.7 (0.5)46.6 (8.5)23.0 (1.5)3.7 (1.2)
    IAA + Trp0.8 (0.4)23.5 (3.1)26.8 (3.7)2.7 (1.1)
    JA + Trp17.2 (1.5)10.5 (2.2)60.3 (4.2)6.9 (0.8)
Seedling
    Light1.2 (0.3)nd2.9 (0.3)2.9 (0.6)5.4 (1.5)
    Dark1.0 (0.3)nd4.7 (0.9)4.0 (0.8)3.0 (1.5)
Leaf
    Mature0.2 (0.02)nd3.8 (1.4)78.6 (40.5)3.8 (1.4)
    Mature wound0.7 (0.02)nd3.4 (0.5)2,064.2 (429)348.5 (19.7)
    Expandingnd5.8 (2.8)16.3 (0.9)18.3 (3.6)7.2 (0.7)
Flowernd2.8 (0.6)62.1 (2.5)47.4 (10.1)8.1 (1.8)
  • a Roots were grown 3 to 4 weeks in MS liquid media followed by 16-h incubation in the same or with IAA or JA at 2 μm and Trp at 25 μm added, as indicated. Seedlings were grown on filter paper with MS liquid media for 5 d in continuous light or continuous dark. Leaves and flowers were from plants grown in soil. Mature leaves were wounded with a hemostat and harvested 1 h later. Expanding leaves were ≤1 cm in length. Detection limit was 0.1 and 0.5 pmol/g FW for JA-Trp and IAA-Trp, respectively.