Table I.

454 sequence read characteristics

Summary of the sequence read characteristics obtained by 454 sequencing of pooled barley chromosomes 1H to 7H (WCAall) and chromosome 1H exclusively (WCA1H).

DatasetNo. Reads Sequenced Before MaskingTotal BasepairsNo. Reads After Masking (%)Median Read LengthM50 LengthN'sNo. Reads with Unique SequencesReads with Unique SequencesNo. Percent >90 MaskedMasked RepeatMasker
bp%%bp %
WCAall381,61799,401,554118,779 (31.1%)2562591.9694,88979.954.374.4
WCA1H3,046,327799,343,261896,421 (29.4%)2582602.44813,91490.856.577.5