Table II.

454 read distribution to barley EST-based markers

Sequence reads from pooled chromosomes 1H to 7H (WCAall) and from a chromosome 1H amplified sequence library were compared with 2,785 unique sequence markers anchored on the genetic map of barley. While for WCAall, an even recovery rate over all seven chromosomes was observed, WCA1H is strongly biased toward chromosome 1H. A total of 89.5% of markers recovered and 90.4% of reads are associated with chromosome 1H.

ChromosomeNo. of markersWCAallWCA1H
No. ReadsNo. Marker DetectedMarkers DetectedNo. ReadsNo. Marker DetectedMarkers DetectedAnchored Reads
%%
1H33237267.81,93229789.590.4
2H46841326.838183.81.8
3H44545357.922132.91.0
4H31432309.616134.10.7
5H49243367.357285.72.7
6H33719175.042298.62.0
7H39728225.531256.31.4
Total2,7852451982,138423