Table I.

Identification of mitochondrial proteins with different abundances between the ndufs4 mutant and wild-type plants

Spot number refers to the annotation in Figure 4. The fold changes and P values were obtained from DeCyder software (GE Healthcare) using three biological replicates. Data from in-gel digestions of the spots were matched using Mascot (Matrix Sciences) to a database of Arabidopsis proteins (The Arabidopsis Information Resource 8). AGI, Arabidopsis Genome Initiative accession number; MOWSE, Molecular Weight Search score derived by Mascot from the MS/MS search; Peptide, number of matching peptides; Coverage, percentage of protein sequence covered by the peptides identified; Localization, subcellular compartment where the protein is located based on MS, GFP, and predictor data according to the SUBA database (Heazlewood et al., 2007).

Spot No.Fold ChangePAGIDescriptionMolecular WeightMOWSEPeptideCoverageLocalization
1−4.180.017At5g37510EMB1467 NDUFS1 EMB1467 (EMBRYO DEFECTIVE1467); NADH dehydrogenase81,1305711421Mitochondria
2−4.320.003
3−4.230.03At5g67590NDUFS4 18-kD subunit17,124140422Mitochondria
4−2.70.01
5−2.60.041At3g03100NDUFA11 NADH:ubiquinone oxidoreductase family protein18,305160322Mitochondria
6−2.140.02
7−2.110.018At3g01500CA1 (CARBONIC ANHYDRASE1); carbonate dehydratase/zinc ion binding36,121130523Chloroplast
8−2.030.009At1g79010NDUFS8-1 NADH-ubiquinone oxidoreductase 23-kD subunit, mitochondrial (TYKY)25,487245630Mitochondria
At1g16700ATMLO14 NDUFS8-2 ATMLO14 (Arabidopsis MILDEW RESISTANCE LOCUS O14); NADH dehydrogenase (ubiquinone)25,361211525Mitochondria
9−2.010.017